ArticleJournal of cell science2016
High resolution microscopy reveals the nuclear shape of budding yeast during cell cycle and in various biological states.
Article in Journal of cell science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- A CRISPR-Cas9-based system for the dose-dependent study of DNA double-strand break sensing and repair.The FEBS journal · 2025Article
- Chromosome Segregation in Closed Mitosis Under an Excess of Nuclear Envelope.Biology of the cell · 2025Article
- FG repeats drive co-clustering of nuclear pores and P granules in the C. elegans germline.Development (Cambridge, England) · 2025Article
- Nuclear basket proteins regulate the distribution and mobility of nuclear pore complexes in budding yeast.Molecular biology of the cell · 2024Article
- SUMOylation at the inner nuclear membrane facilitates nuclear envelope biogenesis during mitosis.The Journal of cell biology · 2023Article
- Polymeric nature of tandemly repeated genes enhances assembly of constitutive heterochromatin in fission yeast.Communications biology · 2023Article
- A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.International journal of molecular sciences · 2023Review
- Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy.Life science alliance · 2022Article
- Quantitative analysis of nuclear pore complex organization inLife science alliance · 2022Article
- Efficient automatic 3D segmentation of cell nuclei for high-content screening.BMC bioinformatics · 2022Article
- The challenge of staying in shape: nuclear size matters.Current genetics · 2021Review
- A new assay capturing chromosome fusions shows a protection trade-off at telomeres and NHEJ vulnerability to low-density ionizing radiation.Nucleic acids research · 2021Article
- Impact of DNA methylation on 3D genome structure.Nature communications · 2021Article
- Article
- High-Resolution Microscopy to Learn the Nuclear Organization of the Living Yeast Cells.Stem cells international · 2021Article
- Regulation of diverse nuclear shapes: pathways working independently, together.Communicative & integrative biology · 2021Review
- Raman Spectroscopy-Based Measurements of Single-Cell Phenotypic Diversity in Microbial Populations.mSphere · 2020Article
- Nucleolar localization of the yeast RNA exosome subunit Rrp44 hints at early pre-rRNA processing as its main function.The Journal of biological chemistry · 2020Article
- Extent and context dependence of pleiotropy revealed by high-throughput single-cell phenotyping.PLoS biology · 2020Article
- Recapitulation of molecular regulators of nuclear motion during cell migration.Cell adhesion & migration · 2019Review
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
How spatial organization of the genome depends on nuclear shape is unknown, mostly because accurate nuclear size and shape measurement is technically challenging. In large cell populations of the yeast Saccharomyces cerevisiae, we assessed the geometry (size and shape) of nuclei in three dimensions with a resolution of 30 nm. We improved an automated fluorescence localization method by implementing a post-acquisition correction of the spherical microscopic aberration along the z-axis, to detect the three dimensional (3D) positions of nuclear pore complexes (NPCs) in the nuclear envelope. Here, we used a method called NucQuant to accurately estimate the geometry of nuclei in 3D throughout the cell cycle. To increase the robustness of the statistics, we aggregated thousands of detected NPCs from a cell population in a single representation using the nucleolus or the spindle pole body (SPB) as references to align nuclei along the same axis. We could detect asymmetric changes of the nucleus associated with modification of nucleolar size. Stereotypical modification of the nucleus toward the nucleolus further confirmed the asymmetric properties of the nuclear envelope.
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