Evidence map›Paper›PMID 27829560›Full record

ArticleBiosensors & bioelectronics2017

Enzyme-free electrochemical immunosensor based on methylene blue and the electro-oxidation of hydrazine on Pt nanoparticles.

Gorachand Dutta, Sureshbabu Nagarajan, Lisa J Lapidus, Peter B Lillehoj

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed, 1 pooled it
4.9field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 1 synthesis or guideline pooled it, 82 citations in OpenAlex.

  1. Pooled it
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  11. Innovations in3 Biotech · 2021
    Review
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  14. Article
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  16. Recent Advances in Electrochemical Immunosensors.Sensors (Basel, Switzerland) · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 2 countries.

Gorachand DuttaDepartment of Mechanical Engineering, Michigan State University, East Lansing, MI 48824, USA.
Sureshbabu NagarajanDepartment of Physics and Astronomy, Michigan State University, East Lansing, MI 48824, USA; Centre for Drug Discovery and Development, Sathyabama University, Chennai, Tamil Nadu 600119, India.
Lisa J LapidusDepartment of Physics and Astronomy, Michigan State University, East Lansing, MI 48824, USA.
Peter B LillehojDepartment of Mechanical Engineering, Michigan State University, East Lansing, MI 48824, USA. Electronic address: lillehoj@egr.msu.edu.
Michigan State University · US

Funding

Rapid in-field malaria diagnosis, prognosis and monitoring using a mobile phoneR01AI113257 · NIAID · RICE UNIVERSITY · PI LILLEHOJ, PETER B · 2015 to 2018
$1.8M
Preventing Protein Aggregation by Controlling Unfolded State DynamicsR01GM100908 · NIGMS · MICHIGAN STATE UNIVERSITY · PI LAPIDUS, LISA J · 2012 to 2016
$1.3M
NIAID NIH HHS R01 AI113257NIGMS NIH HHS R01 GM100908
6 · The paper itself

Abstract

Enzyme-free electrochemical sensors enable rapid, high sensitivity measurements without the limitations associated with enzyme reporters. However, the performance of non-enzymatic electrochemical sensors tends to suffer from slow electrode kinetics and poor signal stability. We report a new enzyme-free electrochemical immunosensor based on a unique competitive detection scheme using methylene blue (MB), hydrazine and platinum nanoparticles (Pt NPs). This scheme is coupled with a robust immunosandwich format employing a MB-labelled detection antibody as a non-enzymatic reporter. In the presence of the target antigen, surface-immobilized MB consumes interfacial hydrazine thereby diminishing the electro-oxidation of hydrazine on Pt NPs. Thus, the concentration of the antigen is directly proportional to the reduction in the electrochemical signal. For proof-of-concept, this sensor was used to detect Plasmodium falciparum histidine-rich protein 2 (PfHRP2), an important malaria biomarker, in unadulterated human saliva samples. Chronocoulometric measurements showed that this platform exhibits pM-range sensitivity, high specificity and good reproducibility, making it well suited for many biosensing applications including noninvasive diagnostic testing.

Indexed as

Antibodies, ProtozoanBiosensing TechniquesElectrochemical TechniquesHistidine-Rich GlycoproteinHumansHydrazinesImmunoassayMalaria, FalciparumMetal NanoparticlesMethylene BlueModels, MolecularPlasmodium falciparumPlatinumProteinsProtozoan ProteinsReproducibility of ResultsAntibodies, ProtozoanHistidine-Rich GlycoproteinhydrazineHydrazinesMethylene BluePlatinumProteinsProtozoan ProteinsElectrochemical immunosensorEnzyme-freeHydrazineMethylene bluePt nanoparticles

Identifiers

PMID27829560
PMCPMC5342929
OpenAlexW2548891411

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.