ArticlePloS one2016
No Evidence for Ionotropic Pheromone Transduction in the Hawkmoth Manduca sexta.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 39 citations in OpenAlex.
- Pheromone-Binding Proteins in Pest Control: From Molecular Insights to Real-World Applications.Journal of agricultural and food chemistry · 2025Review
- Loss of olfaction reduces caterpillar performance and increases susceptibility to a natural enemy.eLife · 2025Article
- Hawkmoth Pheromone Transduction Involves G-Protein-Dependent Phospholipase Cβ Signaling.eNeuro · 2025Article
- Females smell differently: characteristics and significance of the most common olfactory sensilla of female silkmoths.Proceedings. Biological sciences · 2024Article
- Modulation of the NO-cGMP pathway has no effect on olfactory responses in theFrontiers in cellular neuroscience · 2023Article
- Impacts of OrX and cAMP-insensitive Orco to the insect olfactory heteromer activity.Molecular biology reports · 2021Article
- Altered functional properties of the codling moth Orco mutagenized in the intracellular loop-3.Scientific reports · 2021Article
- Functional properties of insect olfactory receptors: ionotropic receptors and odorant receptors.Cell and tissue research · 2021Review
- Developmental and sexual divergence in the olfactory system of the marine insect Clunio marinus.Scientific reports · 2020Article
- Adaptive integrate-and-fire model reproduces the dynamics of olfactory receptor neuron responses in a moth.Journal of the Royal Society, Interface · 2019Article
- Mutagenesis of odorant coreceptorProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Moth olfactory receptor neurons adjust their encoding efficiency to temporal statistics of pheromone fluctuations.PLoS computational biology · 2018Article
- Prior activity of olfactory receptor neurons is required for proper sensory processing and behavior in Drosophila larvae.Scientific reports · 2018Article
- Access to the odor world: olfactory receptors and their role for signal transduction in insects.Cellular and molecular life sciences : CMLS · 2018Review
- Tuning Insect Odorant Receptors.Frontiers in cellular neuroscience · 2018Article
- Insect Pheromone Receptors - Key Elements in Sensing Intraspecific Chemical Signals.Frontiers in cellular neuroscience · 2018Review
- The Two Main Olfactory Receptor Families inFrontiers in cellular neuroscience · 2018Review
- The Diacylglycerol Analogs OAG and DOG Differentially Affect Primary Events of Pheromone Transduction in the HawkmothFrontiers in cellular neuroscience · 2018Article
- Membrane Proteins Mediating Reception and Transduction in Chemosensory Neurons in Mosquitoes.Frontiers in physiology · 2018Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Insect odorant receptors (ORs) are 7-transmembrane receptors with inverse membrane topology. They associate with the conserved ion channel Orco. As chaperon, Orco maintains ORs in cilia and, as pacemaker channel, Orco controls spontaneous activity in olfactory receptor neurons. Odorant binding to ORs opens OR-Orco receptor ion channel complexes in heterologous expression systems. It is unknown, whether this also occurs in vivo. As an alternative to this ionotropic transduction, experimental evidence is accumulating for metabotropic odor transduction, implicating that insect ORs couple to G-proteins. Resulting second messengers gate various ion channels. They generate the sensillum potential that elicits phasic-tonic action potentials (APs) followed by late, long-lasting pheromone responses. Because it is still unclear how and when Orco opens after odor-OR-binding, we used tip recordings to examine in vivo the effects of the Orco antagonist OLC15 and the amilorides MIA and HMA on bombykal transduction in the hawkmoth Manduca sexta. In contrast to OLC15 both amilorides decreased the pheromone-dependent sensillum potential amplitude and the frequency of the phasic AP response. Instead, OLC15 decreased spontaneous activity, increased latencies of phasic-, and decreased frequencies of late, long-lasting pheromone responses Zeitgebertime-dependently. Our results suggest no involvement for Orco in the primary transduction events, in contrast to amiloride-sensitive channels. Instead of an odor-gated ionotropic receptor, Orco rather acts as a voltage- and apparently second messenger-gated pacemaker channel controlling the membrane potential and hence threshold and kinetics of the pheromone response.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.