Evidence map›Paper›PMID 27827819›Full record

ArticleDevelopment (Cambridge, England)2016

PRMT5 is essential for the maintenance of chondrogenic progenitor cells in the limb bud.

Jacqueline L Norrie, Qiang Li, Swanie Co, Bau-Lin Huang, Ding Ding, Jann C Uy, Zhicheng Ji, Susan Mackem, Mark T Bedford, Antonella Galli and 2 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
2.1field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 29 citations in OpenAlex.

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  14. Temporal regulation of chromatin during myoblast differentiation.Seminars in cell & developmental biology · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 5 institutions in 2 countries.

Jacqueline L NorrieDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, USA.
Qiang LiDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, USA.
Swanie CoDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, USA.
Bau-Lin HuangCancer and Developmental Biology Laboratory, CCR, NCI, Frederick, MD 21702, USA.
Ding DingDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Room E3638, Baltimore, MD 21205, USA.
Jann C UyDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, USA.
Zhicheng JiDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Room E3638, Baltimore, MD 21205, USA.
Susan MackemCancer and Developmental Biology Laboratory, CCR, NCI, Frederick, MD 21702, USA.
Mark T BedfordDepartment of Epigenetics & Molecular Carcinogenesis, M.D. Anderson Cancer Center, 1808 Park Road 1C (P.O. Box 389), Smithville, TX 78957, USA.
Antonella GalliWellcome Trust Sanger Institute, Hinxton, Cambridge CB10 1SA, UK.
Hongkai JiDepartment of Biostatistics, Johns Hopkins Bloomberg School of Public Health, 615 North Wolfe Street, Room E3638, Baltimore, MD 21205, USA.
Steven A VokesDepartment of Molecular Biosciences, University of Texas at Austin, 2500 Speedway Stop A4800, Austin, TX 78712, USA svokes@austin.utexas.edu.ORCID 0000-0002-1724-0102
The University of Texas at Austin · USJohns Hopkins University · USFrederick National Laboratory for Cancer Research · USThe University of Texas MD Anderson Cancer Center · USWellcome Sanger Institute · GB

Funding

Late stage signaling centers and Gli3-Hoxd interaction roles in Hedgehog regulated digit morphogenesisZIABC011119 · NCI · DIVISION OF BASIC SCIENCES - NCI · PI MACKEM, SUSAN · 2009 to 2025
$8.9M
Cis-regulatory circuitry underlying Hedgehog mediated limb developmentR01HD073151 · NICHD · UNIVERSITY OF TEXAS AT AUSTIN · PI Steven Alexander Vokes · 2012 to 2026
$5.1M
NICHD NIH HHS R01 HD073151
6 · The paper itself

Abstract

During embryonic development, undifferentiated progenitor cells balance the generation of additional progenitor cells with differentiation. Within the developing limb, cartilage cells differentiate from mesodermal progenitors in an ordered process that results in the specification of the correct number of appropriately sized skeletal elements. The internal pathways by which these cells maintain an undifferentiated state while preserving their capacity to differentiate is unknown. Here, we report that the arginine methyltransferase PRMT5 has a crucial role in maintaining progenitor cells. Mouse embryonic buds lacking PRMT5 have severely truncated bones with wispy digits lacking joints. This novel phenotype is caused by widespread cell death that includes mesodermal progenitor cells that have begun to precociously differentiate into cartilage cells. We propose that PRMT5 maintains progenitor cells through its regulation of Bmp4 Intriguingly, adult and embryonic stem cells also require PRMT5 for maintaining pluripotency, suggesting that similar mechanisms might regulate lineage-restricted progenitor cells during organogenesis.

Indexed as

AnimalsApoptosisBone Morphogenetic Protein 4CartilageCells, CulturedChondrogenesisEmbryonic Stem CellsForelimbLimb BudsMesodermMiceMice, KnockoutProtein-Arginine N-MethyltransferasesSignal TransductionSOX9 Transcription FactorBmp4 protein, mouseBone Morphogenetic Protein 4Prmt5 protein, mouseProtein-Arginine N-MethyltransferasesSox9 protein, mouseSOX9 Transcription FactorBMP4ChondrogenesisLimb developmentPRMT5Progenitor cellsSOX9

Identifiers

PMID27827819
PMCPMC5201029
OpenAlexW2553772989

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.