ArticleMolecular biotechnology2017
Bioengineering of the Plant Culture of Capsicum frutescens with Vanillin Synthase Gene for the Production of Vanillin.
Article in Molecular biotechnology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 35 citations in OpenAlex.
- Chassis selection and metabolic fine-tuning enable efficient in planta betalain production.Plant physiology · 2026Article
- Navigating the challenges of engineering composite specialized metabolite pathways in plants.The Plant journal : for cell and molecular biology · 2025Review
- Genetic Engineering Approaches for the Microbial Production of Vanillin.Biomolecules · 2024Review
- Metabolic engineering of hairy root cultures inFrontiers in bioengineering and biotechnology · 2024Article
- Heterologous mogrosides biosynthesis in cucumber and tomato by genetic manipulation.Communications biology · 2023Article
- Is a spice missing from the recipe? The intra-cellular localization of vanillin biosynthesis needs further investigations.Plant biology (Stuttgart, Germany) · 2023Article
- A whiff of the future: functions of phenylalanine-derived aroma compounds and advances in their industrial production.Plant biotechnology journal · 2022Review
- Synthetic biology of plant natural products: From pathway elucidation to engineered biosynthesis in plant cells.Plant communications · 2021Review
- Plant cell cultures as heterologous bio-factories for secondary metabolite production.Plant communications · 2021Review
- Biochemistry and molecular biology of capsaicinoid biosynthesis: recent advances and perspectives.Plant cell reports · 2019Review
- The Intracellular Localization of the Vanillin Biosynthetic Machinery in Pods of Vanilla planifolia.Plant & cell physiology · 2018Article
- Sourcing VanillinFood technology and biotechnologyReview
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Production of vanillin by bioengineering has gained popularity due to consumer demand toward vanillin produced by biological systems. Natural vanillin from vanilla beans is very expensive to produce compared to its synthetic counterpart. Current bioengineering works mainly involve microbial biotechnology. Therefore, alternative means to the current approaches are constantly being explored. This work describes the use of vanillin synthase (VpVAN), to bioconvert ferulic acid to vanillin in a plant system. The VpVAN enzyme had been shown to directly convert ferulic acid and its glucoside into vanillin and its glucoside, respectively. As the ferulic acid precursor and vanillin were found to be the intermediates in the phenylpropanoid biosynthetic pathway of Capsicum species, this work serves as a proof-of-concept for vanillin production using Capsicum frutescens (C. frutescens or hot chili pepper). The cells of C. frutescens were genetically transformed with a codon optimized VpVAN gene via biolistics. Transformed explants were selected and regenerated into callus. Successful integration of the gene cassette into the plant genome was confirmed by polymerase chain reaction. High-performance liquid chromatography was used to quantify the phenolic compounds detected in the callus tissues. The vanillin content of transformed calli was 0.057% compared to 0.0003% in untransformed calli.
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