ArticleSkeletal muscle2016
Established cell surface markers efficiently isolate highly overlapping populations of skeletal muscle satellite cells by fluorescence-activated cell sorting.
Article in Skeletal muscle, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
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Who cites it
69 citing papers in PubMed, 100 citations in OpenAlex.
- Article
- Transcriptomic atlas of premalignant oral squamous cell carcinoma in an aging mouse model reveals an enhanced immune response and dysregulation of head and neck tissue stem cells.bioRxiv : the preprint server for biology · 2026Article
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- Collagen Scaffold Viscoelasticity Regulates Muscle Cell Phenotype.Advanced healthcare materials · 2026Article
- Modulation of Nudt21 levels reveals dose-dependent roles of alternative polyadenylation in tissue regeneration.Nature communications · 2026Article
- Isolation and Culture of Primary Myoblasts from Humans and Mice.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Endogenous Amphiregulin promotes muscle repair and function through expansion of fibro-adipogenic progenitors during chronicFrontiers in microbiology · 2026Article
- Transdifferentiation of endothelial progenitor cells into rhabdomyosarcoma defined by hedgehog signaling competence.Cell reports · 2025Article
- Muscle-specific gene editing therapy via mammalian fusogen-directed virus-like particles.Nature communications · 2025Article
- A novel protocol for the direct isolation of a highly pure and regenerative population of satellite stem cells.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Muscle Stem Cell Microenvironment and Functions in Muscle Regeneration.Biomolecules · 2025Review
- Deconer: An Evaluation Toolkit for Reference-based Deconvolution Methods Using Gene Expression Data.Genomics, proteomics & bioinformatics · 2025Article
- Soluble RAGE enhances muscle regeneration after cryoinjury in aged and diseased mice.PloS one · 2025Article
- Characterization of Age-Dependent Changes in Skeletal Muscle Repair and Regeneration Using a Mouse Model of Acute Muscle Injury.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Cellular stress and epigenetic regulation in adult stem cells.Life science alliance · 2024Review
- Melatonin ameliorates age-related sarcopenia by inhibiting fibrogenic conversion of satellite cell.Molecular medicine (Cambridge, Mass.) · 2024Article
- Beyond the bulk: overview and novel insights into the dynamics of muscle satellite cells during muscle regeneration.Inflammation and regeneration · 2024Review
- Dissecting the impact of differentiation stage, replicative history, and cell type composition on epigenetic clocks.Stem cell reports · 2024Article
- A dual-color PAX7 and MYF5 in vivo reporter to investigate muscle stem cell heterogeneity in regeneration and aging.Stem cell reports · 2024Article
- Lineage tracing reveals a novel PDGFRβScientific reports · 2024Article
9 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundFluorescent-activated cell sorting (FACS) has enabled the direct isolation of highly enriched skeletal muscle stem cell, or satellite cell, populations from postnatal tissue. Several distinct surface marker panels containing different positively selecting surface antigens have been used to distinguish muscle satellite cells from other non-myogenic cell types. Because functional and transcriptional heterogeneity is known to exist within the satellite cell population, a direct comparison of results obtained in different laboratories has been complicated by a lack of clarity as to whether commonly utilized surface marker combinations select for distinct or overlapping subsets of the satellite cell pool. This study therefore sought to evaluate phenotypic and functional overlap among popular satellite cell sorting paradigms.
methodsUtilizing a transgenic
resultsConsistent with prior reports, each of the commonly used surface marker schemes evaluated here identified a highly enriched satellite cell population, with 89-90 % positivity for Pax7 expression based on zsGreen fluorescence. Distinct surface marker panels were also equivalent in their ability to identify the majority of the satellite cell pool, with 90-93 % of all Pax7-zsGreen positive cells marked by each of the surface marker schemes. The direct comparison among surface marker schemes validated their selection for highly overlapping subsets of cells. Functional analysis in vitro showed no differences in the abilities of cells sorted by these different methods to grow in culture and differentiate.
conclusionsThis study demonstrates the equivalency of several previously published and widely utilized surface marker schemes for isolating a highly purified and myogenically active population of satellite cells from the mouse skeletal muscle, which should facilitate cross-comparison of data across laboratories.
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