ArticlePlasmid2017
An efficient system for the generation of marked genetic mutants in members of the genus Burkholderia.
Article in Plasmid, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 22 citations in OpenAlex.
- Extensive spontaneous genome reduction in Paraburkholderia sabiae.Scientific reports · 2026Article
- Tn-seq profiling reveals that NodS of the beta-rhizobium Paraburkholderia phymatum is detrimental for nodulating soybean.Communications biology · 2024Article
- A CRISPR-Cas-associated transposon system for genome editing inApplied and environmental microbiology · 2024Article
- Identification of a system for hydroxamate xenosiderophore-mediated iron transport inMicrobiology (Reading, England) · 2024Article
- Article
- Phenotype-Guided Comparative Genomics Identifies the Complete Transport Pathway of the Antimicrobial Lasso Peptide Ubonodin inACS chemical biology · 2022Article
- Differential Genetic Strategies of Burkholderia vietnamiensis and Paraburkholderia kururiensis for Root Colonization of Oryza sativa subsp.Applied and environmental microbiology · 2022Article
- Identification of Key Factors for Anoxic Survival ofInternational journal of molecular sciences · 2022Article
- A novel function of the key nitrogen-fixation activator NifA in beta-rhizobia: Repression of bacterial auxin synthesis during symbiosis.Frontiers in plant science · 2022Article
- Methodological tools to study species of the genus Burkholderia.Applied microbiology and biotechnology · 2021Review
- Genetic Diversity of Type 3 Secretion System inFrontiers in microbiology · 2021Article
- Differential Expression ofFrontiers in plant science · 2021Article
- Metabolomics and Transcriptomics Identify Multiple Downstream Targets of Paraburkholderia phymatum σInternational journal of molecular sciences · 2018Article
- Use of Synthetic Hybrid Strains To Determine the Role of Replicon 3 in Virulence of the Burkholderia cepacia Complex.Applied and environmental microbiology · 2017Article
- NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111.PloS one · 2017Article
- Mutations in TwoFrontiers in microbiology · 2017Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
To elucidate the function of a gene in bacteria it is vital that targeted gene inactivation (allelic replacement) can be achieved. Allelic replacement is often carried out by disruption of the gene of interest by insertion of an antibiotic-resistance marker followed by subsequent transfer of the mutant allele to the genome of the host organism in place of the wild-type gene. However, due to their intrinsic resistance to many antibiotics only selected antibiotic-resistance markers can be used in members of the genus Burkholderia, including the Burkholderia cepacia complex (Bcc). Here we describe the construction of improved antibiotic-resistance cassettes that specify resistance to kanamycin, chloramphenicol or trimethoprim effectively in the Bcc and related species. These were then used in combination with and/or to construct a series enhanced suicide vectors, pSHAFT2, pSHAFT3 and pSHAFT-GFP to facilitate effective allelic replacement in the Bcc. Validation of these improved suicide vectors was demonstrated by the genetic inactivation of selected genes in the Bcc species Burkholderia cenocepacia and B. lata, and in the non-Bcc species, B. thailandensis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.