ArticleLipids in health and disease2016
A possible regulatory link between Twist 1 and PPARγ gene regulation in 3T3-L1 adipocytes.
Article in Lipids in health and disease, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Deciphering the interaction between Twist1 and PPARγ during adipocyte differentiation.Cell death & disease · 2023Article
- Roles of Twist1 in lipid and glucose metabolism.Cell communication and signaling : CCS · 2023Review
- OVOL2: an epithelial lineage determiner with emerging roles in energy homeostasis.Trends in cell biology · 2023Review
- Article
- Twist1 downregulation of PGC-1α decreases fatty acid oxidation in tubular epithelial cells, leading to kidney fibrosis.Theranostics · 2022Article
- Elucidating the relationship between dyslipidemia and osteoporosis: A multicenter, prospective cohort study protocol.Frontiers in cardiovascular medicine · 2022Article
- TWIST2 and the PPAR signaling pathway are important in the progression of nonalcoholic steatohepatitis.Lipids in health and disease · 2021Article
- Molecular Phenotyping of White Striping and Wooden Breast Myopathies in Chicken.Frontiers in physiology · 2020Article
- Reduced expression of Twist 1 is protective against insulin resistance of adipocytes and involves mitochondrial dysfunction.Scientific reports · 2018Article
- Identifying potentially common genes between dyslipidemia and osteoporosis using novel analytical approaches.Molecular genetics and genomics : MGG · 2018Article
- Dynamic transcriptome and DNA methylome analyses on longissimus dorsi to identify genes underlying intramuscular fat content in pigs.BMC genomics · 2017Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPeroxisome proliferator-activated receptor γ (PPARγ) is a critical gene that regulates the function of adipocytes. Therefore, studies on the molecular regulation mechanism of PPARγ are important to understand the function of adipose tissue. Twist 1 is another important functional gene in adipose tissue, and hundreds of genes are regulated by Twist 1. The aim of this study was to investigate the regulation of Twist 1 and PPARγ expression in 3T3-L1 mature adipocytes.
methodsWe induced differentiation in 3T3-L1 preadipocytes and examined alterations in Twist 1 and PPARγ expression. We used the PPARγ agonist pioglitazone and the PPARγ antagonist T0070907 to investigate the effect of PPARγ on Twist 1 expression. In addition, we utilized retroviral interference and overexpression of Twist 1 to determine the effects of Twist 1 on PPARγ expression.
resultsThe expression levels of Twist 1 and PPARγ were induced during differentiation in 3T3-L1 adipocytes. Application of either a PPARγ agonist (pioglitazone) or antagonist (T0070907) influenced Twist 1 expression, with up-regulation of Twist 1 under pioglitazone (1 μM, 24 h) and down-regulation of Twist 1 under T0070907 (100 μM, 24 h) exposure. Furthermore, the retroviral interference of Twist 1 decreased the protein and mRNA expression of PPARγ, while Twist 1 overexpression had the opposite effect.
conclusionsThere was a possible regulatory link between Twist 1 and PPARγ in 3T3-L1 mature adipocytes. This regulatory link enhanced the regulation of PPARγ and may be a functional mechanism of Twist 1 regulation of adipocyte physiology and pathology.
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