ArticleThe ISME journal2017
Membrane vesicles in sea water: heterogeneous DNA content and implications for viral abundance estimates.
Article in The ISME journal, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
60 citing papers in PubMed.
- Unveiling Marine Vesicle Uptake in Vibrio spp: Taxonomic and Environmental Insights.MicrobiologyOpen · 2026Article
- Large-scale capsid-mediated mobilisation of bacterial genomic DNA in the gut microbiome.Nature communications · 2026Article
- Bacterial extracellular vesicles exhibit distinct functional potential across biogeographic provinces of the South Pacific Ocean.The ISME journal · 2026Article
- A One Health perspective on bacterial extracellular vesicles as mediators of antimicrobial resistance spread.ISME communications · 2026Article
- Illuminating the newly produced viruses within the virosphere with bioorthogonal noncanonical amino acid tagging and single-virus genomic sequencing technologies.ISME communications · 2026Article
- Limited consensus of marine viral diversity observed across techniques.Environmental microbiome · 2025Article
- Bacterial Extracellular Vesicles: An Emerging Concern in the Field of Environmental Contamination-Characterization, Impacts, and Response Strategies.Microorganisms · 2025Review
- Organic particle scavenging by marine bacteria: influences of bacterial nanoscale surface properties.Applied and environmental microbiology · 2025Article
- Biogenesis of DNA-carrying extracellular vesicles by the dominant human gut methanogenic archaeon.Nature communications · 2025Article
- Distinct horizontal gene transfer potential of extracellular vesicles versus viral-like particles in marine habitats.Nature communications · 2025Article
- Enrichment of horizontally transferred gene clusters in bacterial extracellular vesicles via non lytic mechanisms.The ISME journal · 2025Article
- From microscale to microbial insights: validating high-throughput microvolume extraction (HiMEx) methods for marine microbial ecology.ISME communications · 2025Article
- Marine Delivery Vehicles: Molecular Components and Applications of Bacterial Extracellular Vesicles.Marine drugs · 2024Review
- Exploring Sustainable Agriculture with Nitrogen-Fixing Cyanobacteria and Nanotechnology.Molecules (Basel, Switzerland) · 2024Review
- Capturing of extracellular vesicles derived from single cells ofLab on a chip · 2024Article
- Article
- Bacterial membrane vesicles: orchestrators of interkingdom interactions in microbial communities for environmental adaptation and pathogenic dynamics.Frontiers in immunology · 2024Review
- Breaking the Ice: A Review of Phages in Polar Ecosystems.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Extracellular Vesicles and Bacteriophages: New Directions in Environmental Biocolloid Research.Environmental science & technology · 2023Review
- Extracellular vesicles are the main contributor to the non-viral protected extracellular sequence space.ISME communications · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diverse microbes release membrane-bound extracellular vesicles from their outer surfaces into the surrounding environment. Vesicles are found in numerous habitats including the oceans, where they likely have a variety of functional roles in microbial ecosystems. Extracellular vesicles are known to contain a range of biomolecules including DNA, but the frequency with which DNA is packaged in vesicles is unknown. Here, we examine the quantity and distribution of DNA associated with vesicles released from five different bacteria. The average quantity of double-stranded DNA and size distribution of DNA fragments released within vesicles varies among different taxa. Although some vesicles contain sufficient DNA to be visible following staining with the SYBR fluorescent DNA dyes typically used to enumerate viruses, this represents only a small proportion (<0.01-1%) of vesicles. Thus DNA is packaged heterogeneously within vesicle populations, and it appears that vesicles are likely to be a minor component of SYBR-visible particles in natural sea water compared with viruses. Consistent with this hypothesis, chloroform treatment of coastal and offshore seawater samples reveals that vesicles increase epifluorescence-based particle (viral) counts by less than an order of magnitude and their impact is variable in space and time.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.