ArticleScientific reports2016
Preservation of epithelial progenitor cells from collagenase-digested oral mucosa during ex vivo cultivation.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT03943797 (Cultivated Autologous Oral Mucosal Epithelial Transplantation for the Treatment of Ocular Surface Diseases), which is not on this map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Cultivated Autologous Oral Mucosal Epithelial Transplantation for the Treatment of Ocular Surface Diseases: A Clinical Study
Who cites it
14 citing papers in PubMed, 27 citations in OpenAlex.
- Evaluation of the hydrogels Matrigel, Biogelx, PLTMAx and GelMA in the generation of minilungs from human embryonic stem cells (hESC).Stem cell research & therapy · 2026Article
- Characterization of cultivated nasal mucosal epithelial cell sheets for ocular surface reconstruction: an in vitro and in vivo study.Stem cell research & therapy · 2025Article
- Exploring the differentiation potential of adipose tissue-derived mesenchymal stromal/stem cells and progenitor buccal epithelial cells into urothelial cells.Frontiers in bioengineering and biotechnology · 2025Article
- Endometriosis and Sjögren's syndrome: Bidirectional associations in population-based 15-year retrospective cohorts.Acta obstetricia et gynecologica Scandinavica · 2024Article
- Isolation of Vaginal Epithelial Cells: In Preparation of Autologous Vaginal Tissue Lining for Congenital Absence of Vagina Patients.International journal of molecular sciences · 2023Article
- Novel strategy for primary epithelial cell isolation: Combination of hyaluronidase and collagenase I.Cell proliferation · 2023Article
- Age-dependent oral manifestations of neurofibromatosis type 1: a case-control study.Orphanet journal of rare diseases · 2022Article
- Long-term survival of cultivated oral mucosal epithelial cells in human cornea: generating cell sheets using an animal product-free culture protocol.Stem cell research & therapy · 2021Article
- GMP compliant isolation of mucosal epithelial cells and fibroblasts from biopsy samples for clinical tissue engineering.Scientific reports · 2021Article
- Applications of Human Amniotic Membrane for Tissue Engineering.Membranes · 2021Review
- Article
- Explant culture of oral mucosal epithelial cells for fabricating transplantable epithelial cell sheet.Regenerative therapy · 2019Article
- Technical advantage of recombinant collagenase for isolation of muscle stem cells.Regenerative therapy · 2017Article
- Characterization of Ex Vivo Expanded Oral Mucosal Epithelium Cells on Acellular Porcine Corneal Stroma for Ocular Surface Reconstruction.Journal of ophthalmology · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
12 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To avoid xenogeneic infection, we report a novel protocol for producing animal-derived component-free oral mucosal epithelial cells (OMECs) sheet for transplantation, in which collagenase was used to replace dispase II/trypsin-EDTA for digesting oral mucosal tissue, and human platelet-derived PLTMax to replace fetal bovine serum. The resulting epithelial aggregates were expanded on de-epithelialized amniotic membranes without 3T3 feeder cells, and serum-free EpiLife was used to reduce contamination by submucosal mesenchymal cells. The OMEC sheets thus generated showed similar positive keratin 3/76-positive and keratin 8-negative staining patterns compared with those generated by the original protocol. Colony formation efficiency assay, BrdU label retention assay, and p63 and p75
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.