Evidence map›Paper›PMID 27822563›Full record

ArticleHeliyon2016

Development of an immunoFET biosensor for the detection of biotinylated PCR product.

Wannaporn Muangsuwan, Chamras Promptmas, Wutthinan Jeamsaksiri, Win Bunjongpru, Awirut Srisuwan, Charndet Hruanun, Amporn Poyai, Prapimpun Wongchitrat, Montri Yasawong

Open access · goldAbstract read
In one paragraph

Article in Heliyon, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 23% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. Article
  3. A Novel Nucleic Lateral Flow Assay for ScreeningJournal of microbiology and biotechnology · 2019
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Wannaporn MuangsuwanDepartment of Biochemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Ratchathewi, Bangkok 10400, Thailand.
Chamras PromptmasDepartment of Biomedical Engineering, Faculty of Engineering, Mahidol University, 999 Phutthamonthon 4 Road, Salaya, Phutthamonthon, Nakhon Pathom 73170, Thailand.
Wutthinan JeamsaksiriThai Microelectronics Center (TMEC), 51/4 Moo 1 Suwintawong Road, Wangtakien, Muang, Chachoengsao 24000, Thailand.
Win BunjongpruThai Microelectronics Center (TMEC), 51/4 Moo 1 Suwintawong Road, Wangtakien, Muang, Chachoengsao 24000, Thailand.
Awirut SrisuwanThai Microelectronics Center (TMEC), 51/4 Moo 1 Suwintawong Road, Wangtakien, Muang, Chachoengsao 24000, Thailand.
Charndet HruanunThai Microelectronics Center (TMEC), 51/4 Moo 1 Suwintawong Road, Wangtakien, Muang, Chachoengsao 24000, Thailand.
Amporn PoyaiThai Microelectronics Center (TMEC), 51/4 Moo 1 Suwintawong Road, Wangtakien, Muang, Chachoengsao 24000, Thailand.
Prapimpun WongchitratCenter for Research and Innovation, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
Montri YasawongDepartment of Biochemistry, Faculty of Pharmacy, Mahidol University, 447 Sri-Ayuthaya Road, Ratchathewi, Bangkok 10400, Thailand; Chulabhorn Graduate Institute, 54 Kamphaeng Phet 6, Talat Bang Khen, Lak Si, Bangkok 10210, Thailand.
National Electronics and Computer Technology Center · THMahidol University · TH

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

ImmunoFET (IMFET) biosensor is a simple platform for the detection of biotinylated products of polymerase chain reaction (PCR). Construction of the IMFET biosensor started with adsorption of 1.5 mg/mL of protein A (PA) onto the insulated gate surface of ISFET for 90 min. Next, the immobilized 1/500 dilution of anti-biotin antibody was adsorbed onto the PA layer for 60 min. The IMFET biosensor was subsequently ready for detection of the biotinylated amplicon. The IMFET biosensor showed highly specific binding to the biotinylated PCR product of the

Indexed as

BiochemistryBioengineeringBiotechnologyEngineeringMicrobiology

Identifiers

PMID27822563
PMCPMC5090196
OpenAlexW2545724240

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.