Evidence map›Paper›PMID 27822544›Full record

ArticlemSystems

Multiplexed Metagenomic Deep Sequencing To Analyze the Composition of High-Priority Pathogen Reagents.

Michael R Wilson, Greg Fedewa, Mark D Stenglein, Judith Olejnik, Linda J Rennick, Sham Nambulli, Friederike Feldmann, W Paul Duprex, John H Connor, Elke Mühlberger and 1 more

Abstract read
In one paragraph

Article in mSystems. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed.

  1. Trial
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  4. Genomic and serologic characterization of enterovirus A71 brainstem encephalitis.Neurology(R) neuroimmunology & neuroinflammation · 2020
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Michael R WilsonDepartment of Neurology, University of California, San Francisco, San Francisco, California, USA; Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA.
Greg FedewaIntegrative Program in Quantitative Biology, Bioinformatics, University of California, San Francisco, San Francisco, California, USA.ORCID 0000-0002-3448-3819
Mark D StengleinDepartment of Microbiology, Immunology, and Pathology, Colorado State University, Fort Collins, Colorado, USA.
Judith OlejnikDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.
Linda J RennickDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.
Sham NambulliDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.
Friederike FeldmannRocky Mountain Veterinary Branch, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Hamilton, Montana, USA.
W Paul DuprexDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.
John H ConnorDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.ORCID 0000-0002-8867-7256
Elke MühlbergerDepartment of Microbiology, Boston University School of Medicine, Boston, Massachusetts, USA; National Emerging Infectious Diseases Laboratories, Boston University, Boston, Massachusetts, USA.
Joseph L DeRisiDepartment of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, California, USA; Howard Hughes Medical Institute, Chevy Chase, Maryland, USA.

Funding

Regulatory Compliance CoreUC7AI095321 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI Kelly A Nee · 2014 to 2026
$159.3M
National Center/Emerging Infectous Diseases &BiodefenseUC6AI058618 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI MURPHY, JOHN R. · 2003 to 2006
$141.0M
Clinical and Transtional Science InstituteKL2TR000143 · NCATS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI GRANDIS, JENNIFER RUBIN · 2012 to 2015
$11.9M
BMI Bioinformatics Training GrantsT32GM067547 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI HERNANDEZ, RYAN D. · 2003 to 2022
$6.6M
Development of Near Real-Time, Multiplexed Diagnostics for Viral Hemorrhagic FeveR01AI096159 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI CONNOR, JOHN H · 2011 to 2015
$4.8M
Early Host Immune Response in Protection Against Filovirus InfectionU01AI082954 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI MUHLBERGER, ELKE C · 2009 to 2013
$3.3M
Integrative Program in Complex Biological System (ipCBS)T32EB009383 · NIBIB · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI MARSHALL, WALLACE, WEINER, ORION D · 2009 to 2022
$3.2M
Role for polyamines in Ebola Virus ReplicationR21AI121933 · NIAID · BOSTON UNIVERSITY MEDICAL CAMPUS · PI CONNOR, JOHN H · 2016 to 2017
$452k
NCATS NIH HHS KL2 TR000143NIAID NIH HHS R01 AI096159NIAID NIH HHS R21 AI121933NIAID NIH HHS U01 AI082954NIAID NIH HHS UC6 AI058618NIAID NIH HHS UC7 AI095321NIBIB NIH HHS T32 EB009383NIGMS NIH HHS T32 GM067547
6 · The paper itself

Abstract

Laboratories studying high-priority pathogens need comprehensive methods to confirm microbial species and strains while also detecting contamination. Metagenomic deep sequencing (MDS) inventories nucleic acids present in laboratory stocks, providing an unbiased assessment of pathogen identity, the extent of genomic variation, and the presence of contaminants. Double-stranded cDNA MDS libraries were constructed from RNA extracted from

Indexed as

metagenomicspathogen trackingphylogenetic analysis

Identifiers

PMID27822544
PMCPMC5069959

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.