Evidence map›Paper›PMID 27822330›Full record

ArticleBiomicrofluidics2016

Adhesion patterning by a novel air-lock technique enables localization and

S Sakamoto, K O Okeyo, S Yamazaki, O Kurosawa, H Oana, H Kotera, M Washizu

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biomicrofluidics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 35% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

S SakamotoDepartment of Bioengineering, School of Engineering, The University of Tokyo , Tokyo 113-3656, Japan.
K O OkeyoDepartment of Mechanical Engineering, School of Engineering, The University of Tokyo , Tokyo 113-3656, Japan.ORCID 0000-0001-8027-8069
S YamazakiCenter for Stem Cell Therapy, The Institute of Medical Science, The University of Tokyo , Tokyo 113-3656, Japan.
O KurosawaDepartment of Bioengineering, School of Engineering, The University of Tokyo , Tokyo 113-3656, Japan.
H OanaDepartment of Mechanical Engineering, School of Engineering, The University of Tokyo , Tokyo 113-3656, Japan.
H KoteraDepartment of Microengineering, School of Engineering, Kyoto University , Kyoto 606-8501, Japan.
M Washizu
The University of Tokyo · JPKyoto University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Although fusion of somatic cells with embryonic stem (ES) cells has been shown to induce reprogramming, single-cell level details of the transitory phenotypic changes that occur during fusion-based reprogramming are still lacking. Our group previously reported on the technique of one-to-one electrofusion via micro-slits in a microfluidic platform. In this study, we focused on developing a novel air-lock patterning technique for creating localized adhesion zones around the micro-slits for cell localization and real-time imaging of post fusion events with a single-cell resolution. Mouse embryonic fibroblasts (MEF) were fused individually with mouse ES cells using a polydimethylsiloxane (PDMS) fusion chip consisting of two feeder channels with a separating wall containing an array of micro-slits (slit width ∼3

Identifiers

PMID27822330
PMCPMC5085977
OpenAlexW2545286687

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.