ArticlePlant methods2016
A rapid and cost-effective fluorescence detection in tube (FDIT) method to analyze protein phosphorylation.
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 18 citations in OpenAlex.
- Magnaporthe oryzae MoPh1 perceives ER stress and promotes adaptive responses via a plasma membrane-to-vacuole pathway.Nature communications · 2026Article
- Suppressing wheat sucrose phosphate synthase 1-B protects wheat against stripe rust.Journal of advanced research · 2025Article
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- Autophagy and cell wall integrity pathways coordinately regulate the development and pathogenicity through MoAtg4 phosphorylation in Magnaporthe oryzae.PLoS pathogens · 2024Article
- Suppression of ZEAXANTHIN EPOXIDASE 1 restricts stripe rust growth in wheat.Plant communications · 2023Article
- Homeostasis of cell wall integrity pathway phosphorylation is required for the growth and pathogenicity of Magnaporthe oryzae.Molecular plant pathology · 2022Article
- The rice blast fungus MoRgs1 functioning in cAMP signaling and pathogenicity is regulated by casein kinase MoCk2 phosphorylation and modulated by membrane protein MoEmc2.PLoS pathogens · 2021Article
- Auxilin-like protein MoSwa2 promotes effector secretion and virulence as a clathrin uncoating factor in the rice blast fungus Magnaporthe oryzae.The New phytologist · 2021Article
- A self-balancing circuit centered on MoOsm1 kinase governs adaptive responses to host-derived ROS ineLife · 2020Article
- Shedding light on autophagy coordinating with cell wall integrity signaling to govern pathogenicity ofAutophagy · 2020Article
- A split ubiquitin system to reveal topology and released peptides of membrane proteins.BMC biotechnology · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundProtein phosphorylation is one of the most important post-translational modifications catalyzed by protein kinases in living organisms. The advance of genome sequencing provided the information of protein kinase families in many organisms, including both model and non-model plants. The development of proteomics technologies also enabled scientists to efficiently reveal a large number of protein phosphorylations of an organism. However, kinases and phosphorylation targets are still to be connected to illustrate the complicated network in life.
resultsHere we adapted Pro-Q
conclusionThe FDIT method saves up to 95% of the dye consumed in a gel staining method. The FDIT method is remarkably quick, highly reproducible, unambiguous and capable to be scaled up to dozens of samples. The FDIT method could serve as a simple and sensitive alternative procedure to determine protein kinase reactions with zero radiation exposure, as a supplementation to other widely used radioactive and in-gel assays.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.