ArticleProceedings of the National Academy of Sciences of the United States of America2016
Design of a molecular support for cryo-EM structure determination.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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Who cites it
40 citing papers in PubMed.
- DNA-Guided Robust Single-Protein Electronic Readout.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
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- Functionalizing DNA Origami by Triplex-Directed Site-Specific Photo-Cross-Linking.Journal of the American Chemical Society · 2024Article
- DNA origami: a tool to evaluate and harness transcription factors.Journal of molecular medicine (Berlin, Germany) · 2023Review
- Cryo-EM structure determination of small therapeutic protein targets at 3 Å-resolution using a rigid imaging scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Advancing the Utility of DNA Origami Technique through Enhanced Stability of DNA-Origami-Based Assemblies.Bioconjugate chemistry · 2023Review
- Automated design of 3D DNA origami with non-rasterized 2D curvature.Science advances · 2022Article
- The wending rhombus: Self-assembling 3D DNA crystals.Biophysical journal · 2022Review
- Protein Design: From the Aspect of Water Solubility and Stability.Chemical reviews · 2022Review
- Pharmaceutical applications of framework nucleic acids.Acta pharmaceutica Sinica. B · 2022Review
- Structural basis of reactivation of oncogenic p53 mutants by a small molecule: methylene quinuclidinone (MQ).Nature communications · 2021Article
- DNA origami nano-mechanics.Chemical Society reviews · 2021Review
- The role of SAXS and molecular simulations in 3D structure elucidation of a DNA aptamer against lung cancer.Molecular therapy. Nucleic acids · 2021Article
- Obtaining Precise Molecular Information via DNA Nanotechnology.Membranes · 2021Review
- Article
- Designed and biologically active protein lattices.Nature communications · 2021Article
- DNA origami signposts for identifying proteins on cell membranes by electron cryotomography.Cell · 2021Article
- Nuclease resistance of DNA nanostructures.Nature reviews. Chemistry · 2021Review
- Megabodies expand the nanobody toolkit for protein structure determination by single-particle cryo-EM.Nature methods · 2021Article
- Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution.Nature communications · 2020Article
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Authors and funding
8 authors.
Funding
Abstract
Despite the recent rapid progress in cryo-electron microscopy (cryo-EM), there still exist ample opportunities for improvement in sample preparation. Macromolecular complexes may disassociate or adopt nonrandom orientations against the extended air-water interface that exists for a short time before the sample is frozen. We designed a hollow support structure using 3D DNA origami to protect complexes from the detrimental effects of cryo-EM sample preparation. For a first proof-of-principle, we concentrated on the transcription factor p53, which binds to specific DNA sequences on double-stranded DNA. The support structures spontaneously form monolayers of preoriented particles in a thin film of water, and offer advantages in particle picking and sorting. By controlling the position of the binding sequence on a single helix that spans the hollow support structure, we also sought to control the orientation of individual p53 complexes. Although the latter did not yet yield the desired results, the support structures did provide partial information about the relative orientations of individual p53 complexes. We used this information to calculate a tomographic 3D reconstruction, and refined this structure to a final resolution of ∼15 Å. This structure settles an ongoing debate about the symmetry of the p53 tetramer bound to DNA.
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