ArticleProceedings of the National Academy of Sciences of the United States of America2016
Disease resistance through impairment of α-SNAP-NSF interaction and vesicular trafficking by soybean Rhg1.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
53 citing papers in PubMed, 97 citations in OpenAlex.
- AATRhg1 is a tonoplast protein that alters amino acid, metabolic, and defense responses and nematode resistance.Plant physiology · 2026Article
- Molecular choreography of vesicle trafficking: Exocyst-SNARE interplay in plant defense.Journal of advanced research · 2026Review
- Time- and Genotype-Dependent Root-Transcriptomic Responses of Soybean to Combined Soybean Aphid and Soybean Cyst Nematode Infestation.Plants (Basel, Switzerland) · 2026Article
- A Highly Conserved SNARE-Associated Protein Enhances Plant Immunity by Regulating Vesicle Trafficking.Plant biotechnology journal · 2026Article
- GmSNAP14: a key contributor to soybean cyst nematode resistance in soybean.The New phytologist · 2026Article
- Light-Signalling Regulators GmSTF3/4 Enhance Soybean Susceptibility to Cyst Nematodes via Activation of Sugar Transporters.Plant biotechnology journal · 2025Article
- Identification of candidate genes associated with resistance to Heterodera sojae in soybean.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- The AP2/ERF transcription factor GmTINY mediates ethylene regulation of Rhg1-conferred resistance against soybean cyst nematode.Plant communications · 2025Article
- Gene Localization and Functional Validation ofPlants (Basel, Switzerland) · 2025Article
- Impact of Rhg1 copy number variation on a soybean cyst nematode resistance transcriptional network.G3 (Bethesda, Md.) · 2024Article
- Copy number variations at theFrontiers in plant science · 2024Article
- Deep Learning Model for Classifying and Evaluating Soybean Leaf Disease Damage.International journal of molecular sciences · 2023Article
- No Pairwise Interactions of GmSNAP18, GmSHMT08 and AtPR1 with SuppressedPlants (Basel, Switzerland) · 2023Article
- Loss-of-function of an α-SNAP gene confers resistance to soybean cyst nematode.Nature communications · 2023Article
- Research progress on the functional study of host resistance-related genes against Heterodera glycines.Crop health · 2023Review
- Single Nematode Transcriptomic Analysis, Using Long-Read Technology, Reveals Two Novel Virulence Gene Candidates in the Soybean Cyst Nematode,International journal of molecular sciences · 2023Article
- Soybean transporter AAT Rhg1 abundance increases along the nematode migration path and impacts vesiculation and ROS.Plant physiology · 2023Article
- Beet cyst nematode HsSNARE1 interacts with both AtSNAP2 and AtPR1 and promotes disease in Arabidopsis.Journal of advanced research · 2023Article
- Cataloging SCN resistance loci in North American public soybean breeding programs.Frontiers in plant science · 2023Article
- Large-scale data mining pipeline for identifying novel soybean genes involved in resistance against the soybean cyst nematode.Frontiers in bioinformatics · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
α-SNAP [soluble NSF (N-ethylmaleimide-sensitive factor) attachment protein] and NSF proteins are conserved across eukaryotes and sustain cellular vesicle trafficking by mediating disassembly and reuse of SNARE protein complexes, which facilitate fusion of vesicles to target membranes. However, certain haplotypes of the Rhg1 (resistance to Heterodera glycines 1) locus of soybean possess multiple repeat copies of an α-SNAP gene (Glyma.18G022500) that encodes atypical amino acids at a highly conserved functional site. These Rhg1 loci mediate resistance to soybean cyst nematode (SCN; H. glycines), the most economically damaging pathogen of soybeans worldwide. Rhg1 is widely used in agriculture, but the mechanisms of Rhg1 disease resistance have remained unclear. In the present study, we found that the resistance-type Rhg1 α-SNAP is defective in interaction with NSF. Elevated in planta expression of resistance-type Rhg1 α-SNAPs depleted the abundance of SNARE-recycling 20S complexes, disrupted vesicle trafficking, induced elevated abundance of NSF, and caused cytotoxicity. Soybean, due to ancient genome duplication events, carries other loci that encode canonical (wild-type) α-SNAPs. Expression of these α-SNAPs counteracted the cytotoxicity of resistance-type Rhg1 α-SNAPs. For successful growth and reproduction, SCN dramatically reprograms a set of plant root cells and must sustain this sedentary feeding site for 2-4 weeks. Immunoblots and electron microscopy immunolocalization revealed that resistance-type α-SNAPs specifically hyperaccumulate relative to wild-type α-SNAPs at the nematode feeding site, promoting the demise of this biotrophic interface. The paradigm of disease resistance through a dysfunctional variant of an essential gene may be applicable to other plant-pathogen interactions.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.