ArticleProceedings of the National Academy of Sciences of the United States of America2016
Illuminating a plant's tissue-specific metabolic diversity using computational metabolomics and information theory.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed.
- Genome-wide associations of leaf spectral variation in MAGIC lines of Nicotiana attenuata.Ecology · 2026Article
- Chromosome-level genome assemblies of Nicotiana attenuata (coyote tobacco) and Nicotiana obtusifolia (desert tobacco).Scientific data · 2026Article
- Roots of Indian heliotrope (Heliotropium indicum) produce simple pyrrolizidine alkaloids using the same homospermidine oxidase involved in biosynthesis of complex pyrrolizidine alkaloids in aerial parts.Plant biology (Stuttgart, Germany) · 2025Article
- A Novel Approach for Comparing Selected Metabolites in Citrus Leaves and Fruits Across Datasets.Plants (Basel, Switzerland) · 2025Article
- Untargeted metabolomics reveals anion and organ-specific metabolic responses of salinity tolerance in willow.The Plant journal : for cell and molecular biology · 2025Article
- Plant sesquiterpene lactones.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2024Review
- Phytochemical drivers of insect herbivory: a functional toolbox to support agroecological diversification.Royal Society open science · 2024Article
- Dynamic relationships among pathways producing hydrocarbons and fatty acids of maize silk cuticular waxes.Plant physiology · 2024Article
- Nitrogen and sulfur for phosphorus: Lipidome adaptation of anaerobic sulfate-reducing bacteria in phosphorus-deprived conditions.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Selective lipid recruitment by an archaeal DPANN symbiont from its host.Nature communications · 2024Article
- Estimating Transcriptome Diversity and Specialization inPlants (Basel, Switzerland) · 2024Article
- Article
- Metabolome Profiling ofInternational journal of molecular sciences · 2023Article
- Evolutionary metabolomics of specialized metabolism diversification in the genusScience advances · 2023Article
- Integrative omics approaches for biosynthetic pathway discovery in plants.Natural product reports · 2022Review
- Expanding the Coverage of Metabolic Landscape in Cultivated Rice with Integrated Computational Approaches.Genomics, proteomics & bioinformatics · 2022Article
- Harmonizing biosynthesis with post-ingestive modifications to understand the ecological functions of plant natural products.Natural product reports · 2022Review
- Using interdisciplinary, phylogeny-guided approaches to understand the evolution of plant metabolism.Plant molecular biology · 2022Review
- Identification of Gene Biomarkers for Tigilanol Tiglate Content inMolecules (Basel, Switzerland) · 2022Article
- Aerial and underground organs display specific metabolic strategies to cope with water stress under rising atmospheric COPhysiologia plantarum · 2022Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Secondary metabolite diversity is considered an important fitness determinant for plants' biotic and abiotic interactions in nature. This diversity can be examined in two dimensions. The first one considers metabolite diversity across plant species. A second way of looking at this diversity is by considering the tissue-specific localization of pathways underlying secondary metabolism within a plant. Although these cross-tissue metabolite variations are increasingly regarded as important readouts of tissue-level gene function and regulatory processes, they have rarely been comprehensively explored by nontargeted metabolomics. As such, important questions have remained superficially addressed. For instance, which tissues exhibit prevalent signatures of metabolic specialization? Reciprocally, which metabolites contribute most to this tissue specialization in contrast to those metabolites exhibiting housekeeping characteristics? Here, we explore tissue-level metabolic specialization in Nicotiana attenuata, an ecological model with rich secondary metabolism, by combining tissue-wide nontargeted mass spectral data acquisition, information theory analysis, and tandem MS (MS/MS) molecular networks. This analysis was conducted for two different methanolic extracts of 14 tissues and deconvoluted 895 nonredundant MS/MS spectra. Using information theory analysis, anthers were found to harbor the most specialized metabolome, and most unique metabolites of anthers and other tissues were annotated through MS/MS molecular networks. Tissue-metabolite association maps were used to predict tissue-specific gene functions. Predictions for the function of two UDP-glycosyltransferases in flavonoid metabolism were confirmed by virus-induced gene silencing. The present workflow allows biologists to amortize the vast amount of data produced by modern MS instrumentation in their quest to understand gene function.
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