Evidence map›Paper›PMID 27821695›Full record

ArticleThe American journal of tropical medicine and hygiene2017

Development of a PCR Assay for Diagnosing Trematode (Opisthorchis and Haplorchis) Infections in Human Stools.

Pheophet Lamaningao, Seiji Kanda, Sakhone Laimanivong, Takaki Shimono, Andrew Waleluma Darcy, Amphay Phyaluanglath, Nobuyuki Mishima, Toshimasa Nishiyama

Abstract read
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Review
  3. Article
  4. Diagnosis of Human Trematode Infections.Advances in experimental medicine and biology · 2024
    Review
  5. Performance of Mini ParasepParasites & vectors · 2022
    Article
  6. Article
  7. Improvement of a PCR-based method for the detection ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2021
    Article
  8. Article
  9. Review
  10. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Pheophet LamaningaoDepartment of Public Health, Kansai Medical University, Osaka, Japan. kandas@hirakata.kmu.ac.jp.
Seiji KandaDepartment of Public Health, Kansai Medical University, Osaka, Japan. kandas@hirakata.kmu.ac.jp.
Sakhone LaimanivongCenter of Malariology, Parasitology, and Entomology, Ministry of Health, Vientiane, Lao People's Democratic Republic.
Takaki ShimonoDepartment of Public Health, Kansai Medical University, Osaka, Japan.
Andrew Waleluma DarcyDepartment of Public Health, Kansai Medical University, Osaka, Japan.
Amphay PhyaluanglathClinical Laboratory, Mahosot Hospital, Ministry of Health, Vientiane, Lao People's Democratic Republic.
Nobuyuki MishimaDepartment of Public Health, Kansai Medical University, Osaka, Japan.
Toshimasa NishiyamaDepartment of Public Health, Kansai Medical University, Osaka, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We developed a combined conventional polymerase chain reaction (PCR) and real-time PCR (qPCR)-based assay for detecting and discriminating between Opisthorchis viverrini and Haplorchis taichui parasite infections. The first PCR amplifies the mitochondrial cytochrome c oxidase subunit I (COI) genes of parasites, and differential diagnosis is achieved by performing qPCR with specific primers and SYBR Green I. The detection limit of the assay was found to be 2.0 × 10

Indexed as

AnimalsBendamustine HydrochlorideElectron Transport Complex IVFecesGene Expression Regulation, EnzymologicHumansMitochondriaOpisthorchisPolymerase Chain ReactionSensitivity and SpecificityTrematode InfectionsBendamustine HydrochlorideElectron Transport Complex IV

Identifiers

PMID27821695
PMCPMC5239698

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.