ArticleMalaria journal2016
Comparison of molecular quantification of Plasmodium falciparum gametocytes by Pfs25 qRT-PCR and QT-NASBA in relation to mosquito infectivity.
Article in Malaria journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 29 citations in OpenAlex.
- Plasmodium falciparum gametocyte dynamics after pyronaridine-artesunate or artemether-lumefantrine treatment.Malaria journal · 2018Trial
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- A Molecular Assay to Quantify Male and Female Plasmodium falciparum Gametocytes: Results From 2 Randomized Controlled Trials Using Primaquine for Gametocyte Clearance.The Journal of infectious diseases · 2017Trial
- Molecular Markers for Sensitive Detection ofThe American journal of tropical medicine and hygiene · 2017Trial
- Characterizing the Circulation of Gametocytes and Mosquito Infectivity in Induced Blood Stage Malaria.The Journal of infectious diseases · 2026Article
- The Effect of Artemether-Lumefantrine Combined with a Single Dose of Primaquine onTropical medicine and infectious disease · 2026Article
- Histamine Ingestion byBiomolecules · 2021Article
- Characterization of Naturally Acquired Immunity to a Panel of Antigens Expressed in MatureFrontiers in cellular and infection microbiology · 2021Article
- Detection of remaining Plasmodium DNA and gametocytes during follow up after curative malaria treatment among returned travellers in Norway.Malaria journal · 2020Article
- Assays for quantification of male and female gametocytes in human blood by qRT-PCR in the absence of pure sex-specific gametocyte standards.Malaria journal · 2020Article
- Molecular detection and quantification of Plasmodium falciparum gametocytes carriage in used RDTs in malaria elimination settings in northern Senegal.Malaria journal · 2020Article
- qRT-PCR versus IFA-based Quantification of Male and Female Gametocytes in Low-Density Plasmodium falciparum Infections and Their Relevance for Transmission.The Journal of infectious diseases · 2020Article
- HIV-1 Infection Is Associated With Increased Prevalence and Abundance ofFrontiers in cellular and infection microbiology · 2020Article
- Antibody Responses to Crude Gametocyte Extract PredictFrontiers in immunology · 2020Article
- A multiplex assay for the sensitive detection and quantification of male and female Plasmodium falciparum gametocytes.Malaria journal · 2018Article
- Article
- Detection of Plasmodium falciparum male and female gametocytes and determination of parasite sex ratio in human endemic populations by novel, cheap and robust RTqPCR assays.Malaria journal · 2017Article
- Reassessment of asymptomatic carriers of Plasmodium spp. in an endemic area with a very low incidence of malaria in extra-Amazonian Brazil.Malaria journal · 2017Article
- Examining the human infectious reservoir for Plasmodium falciparum malaria in areas of differing transmission intensity.Nature communications · 2017Article
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Authors and funding
20 authors at 7 institutions in 6 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantifying gametocyte densities in natural malaria infections is important to estimate malaria transmission potential. Two molecular methods (Pfs25 mRNA quantitative reverse transcriptase PCR (qRT-PCR) and Pfs25 mRNA quantitative nucleic acid sequence based amplification (QT-NASBA)) are commonly used to determine gametocyte densities in clinical and epidemiological studies and allow gametocyte detection at densities below the microscopic threshold for detection. Here, reproducibility of these measurements and the association between estimated gametocyte densities and mosquito infection rates were compared.
methodsTo quantify intra- and inter-assay variation of QT-NASBA and qRT-PCR, a series of experiments was performed using culture-derived mature Plasmodium falciparum gametocytes from three different parasite isolates (NF54, NF135, NF166). Pfs25 mRNA levels were also determined in samples from clinical trials in Mali and Burkina Faso using both methods. Agreement between the two methods and association with mosquito infection rates in membrane feeding assays were assessed.
resultsIntra- and inter-assay variability was larger in QT-NASBA compared to qRT-PCR, particularly at low gametocyte densities (< 1 gametocyte per μL). Logistic models, including log-transformed gametocytaemia estimated by QT-NASBA, explained variability in mosquito feeding experiment results as well as log-transformed gametocytaemia by qRT-PCR (marginal R
conclusionsThe experiments indicate that both qRT-PCR and QT-NASBA are of value for quantifying mature gametocytes in samples collected in field studies. For both assays, estimated gametocyte densities correlated well with mosquito infection rates. QT-NASBA is less reproducible than qRT-PCR, particularly for low gametocyte densities.
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