ArticleBMC microbiology2016
Determination of virulence and antibiotic resistance pattern of biofilm producing Listeria species isolated from retail raw milk.
Article in BMC microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
19 citing papers in PubMed, 2 syntheses or guidelines pooled it, 45 citations in OpenAlex.
- Antimicrobial-resistant foodborne pathogens in the Middle East: a systematic review.Environmental science and pollution research international · 2021Pooled it
- Epidemiology of Listeria monocytogenes prevalence in foods, animals and human origin from Iran: a systematic review and meta-analysis.BMC public health · 2018Pooled it
- Digestibility, ruminal enzyme activity, fermentation characteristics, and selected blood biochemical parameters in calves supplemented with humic acid.Frontiers in veterinary science · 2025Article
- Detection and Molecular Characterization ofVeterinary medicine international · 2025Article
- Foodborne bacteria in milk and milk products along the water buffalo milk chain in Bangladesh.Scientific reports · 2024Article
- Prevalence of Listeria monocytogenes in milk in Africa: a generalized logistic mixed-effects and meta-regression modelling.Scientific reports · 2023Article
- Antibacterial and anti-biofilm activities of probioticFrontiers in microbiology · 2023Article
- Newly Emerging MDRInfection and drug resistance · 2022Article
- First substantiation of clinico-molecular investigation of pathogenicIranian journal of veterinary research · 2022Article
- Bioactivity of Essential Oils for Mitigation ofFoods (Basel, Switzerland) · 2021Article
- Conditions of In Vitro Biofilm Formation by Serogroups ofFoods (Basel, Switzerland) · 2021Article
- Virulence Characterization ofPathogens (Basel, Switzerland) · 2020Article
- Association among biofilm formation, virulence gene expression, and antibiotic resistance in Proteus mirabilis isolates from diarrhetic animals in Northeast China.BMC veterinary research · 2020Article
- Contamination Pathways can Be Traced along the Poultry Processing Chain by Whole Genome Sequencing ofMicroorganisms · 2020Article
- Prevalence, Pathogenicity, Virulence, Antibiotic Resistance, and Phylogenetic Analysis of Biofilm-ProducingPathogens (Basel, Switzerland) · 2019Article
- PersistentPathogens (Basel, Switzerland) · 2019Article
- Inhibitory effect of bacteriocins from enterococci on developing and preformed biofilms of Listeria monocytogenes, Listeria ivanovii and Listeria innocua.World journal of microbiology & biotechnology · 2019Article
- Insights Into the Bovine Milk Microbiota in Dairy Farms With Different Incidence Rates of Subclinical Mastitis.Frontiers in microbiology · 2018Article
- A Comprehensive Review of Common Bacterial, Parasitic and Viral Zoonoses at the Human-Animal Interface in Egypt.Pathogens (Basel, Switzerland) · 2017Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOne of the foodborne pathogens is Listeria monocytogenes, which causes serious invasive illness in elderly and immunocompromised patients, pregnant women, newborns and infants ranking second after salmonellosis because of its high case fatality rate. Listerial cow mastitis marked by abnormal milk, increased cell counts and reduced production has not been reported. Therefore, apparently healthy cows can be reservoirs of L. monocytogenes. A number of 203 udder milk samples from apparently healthy animals (buffalo, n = 100; cow, n = 103) were collected and tested for Listeria. Isolated colonies on the PALCAM agar were Listeria species confirmed according to their biochemical and the Christie-Atkins-Munch-Petersen (CAMP) reactions. The Listeria species pathogenicity of was tested by phosphatidylinositol-specific phospholipase C, DL-alanine-β-naphthylamide HCl, Dalanine-p-nitroanilide tests, chick embryo, mice inoculation tests, Vero cell cytotoxicity and biofilm formation. The virulence-associated genes, hlyA, plcB, actA and iap associated with Listeria were molecularly assayed.
resultsThe 17 isolated Listeria spp. represented a prevalence rate of 8.4 %. Of these 3 (1.4 %), 2 (1 %), 5 (2.5 %), 4 (2 %) and 3 (1.5 %) were confirmed as L. monocytogenes, L. innocua, L. welshimeri, L. seelegeri, respectively. While the L. monocytogenes isolate displayed all the four virulence-associated genes, L. seelegeri carried the hlyA gene only. The L. monocytogenes had a strong in vitro affinity to form a biofilm, in particular serotype 4 which is associated with human infections. L. monocytogenes showed resistance for 9/27 antibiotics.
conclusionsThe biofilm forming capability of the Listeria spps. makes them particularly successful in colonizing surfaces within the host thus being responsible for persistence infections and due to their antimicrobial resistant phenotype that this structure confers. In addition, strains belonging to serotypes associated with human infections and characterized by pathogenic potential (serotype 4) are capable to persist within the processing plants forming biofilm and thus being a medical hazard.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.