ArticleNature chemical biology2017
Genome-wide genetic screening with chemically mutagenized haploid embryonic stem cells.
Article in Nature chemical biology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Comprehensive CRISPR/Cas9-based mutagenesis identifies single-amino acid substitutions that abrogate SPEN function in X inactivation.Nature communications · 2026Article
- Crimean-Congo haemorrhagic fever virus uses LDLR to bind and enter host cells.Nature microbiology · 2024Article
- Development and application of haploid embryonic stem cells.Stem cell research & therapy · 2024Review
- Revolutionizing DNA repair research and cancer therapy with CRISPR-Cas screens.Nature reviews. Molecular cell biology · 2023Review
- Functional E3 ligase hotspots and resistance mechanisms to small-molecule degraders.Nature chemical biology · 2023Article
- SDR enzymes oxidize specific lipidic alkynylcarbinols into cytotoxic protein-reactive species.eLife · 2022Article
- Article
- Directed evolution in mammalian cells.Nature methods · 2021Review
- Loss of Mediator complex subunit 13 (MED13) promotes resistance to alkylation through cyclin D1 upregulation.Nucleic acids research · 2021Article
- Applications ofStem cells international · 2021Review
- Moving forward with forward genetics: A summary of the INFRAFRONTIER Forward Genetics Panel Discussion.F1000Research · 2021Article
- Current advances in haploid stem cells.Protein & cell · 2020Review
- The milestone of genetic screening: Mammalian haploid cells.Computational and structural biotechnology journal · 2020Review
- Coupling chemical mutagenesis to next generation sequencing for the identification of drug resistance mutations in Leishmania.Nature communications · 2019Article
- Derivation and maintenance of mouse haploid embryonic stem cells.Nature protocols · 2019Article
- Mammalian haploid stem cells: establishment, engineering and applications.Cellular and molecular life sciences : CMLS · 2019Review
- Gain- and Loss-of-Function Screens Coupled to Next-Generation Sequencing for Antibiotic Mode of Action and Resistance Studies inAntimicrobial agents and chemotherapy · 2019Article
- Genome-wide prediction of synthetic rescue mediators of resistance to targeted and immunotherapy.Molecular systems biology · 2019Article
- Article
- Detection of functional protein domains by unbiased genome-wide forward genetic screening.Scientific reports · 2018Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
In model organisms, classical genetic screening via random mutagenesis provides key insights into the molecular bases of genetic interactions, helping to define synthetic lethality, synthetic viability and drug-resistance mechanisms. The limited genetic tractability of diploid mammalian cells, however, precludes this approach. Here, we demonstrate the feasibility of classical genetic screening in mammalian systems by using haploid cells, chemical mutagenesis and next-generation sequencing, providing a new tool to explore mammalian genetic interactions.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.