ArticleScientific reports2016
TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Review
- The mosaicism of Cas-induced mutations and pleiotropic effects of scarlet gene in an emerging model system.Heredity · 2025Article
- Spatiotemporal control of transgene expression using an infrared laser in the crustacean Daphnia magna.Scientific reports · 2024Article
- Identification of gene isoforms and their switching events between male and female embryos of the parthenogenetic crustacean Daphnia magna.Scientific reports · 2024Article
- Efficient CRISPR genome editing and integrative genomic analyses reveal the mosaicism of Cas-induced mutations and pleiotropic effects ofbioRxiv : the preprint server for biology · 2024Article
- Review
- Regulation of Doublesex1 Expression for Environmental Sex Determination in the Cladoceran Crustacean Daphnia.Frontiers in cell and developmental biology · 2022Review
- Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna.PloS one · 2020Article
- Article
- Genomic integration and ligand-dependent activation of the human estrogen receptor α in the crustacean Daphnia magna.PloS one · 2018Article
- Mapping the expression of the sex determining factor Doublesex1 in Daphnia magna using a knock-in reporter.Scientific reports · 2017Article
- Co-option of the bZIP transcription factor Vrille as the activator of Doublesex1 in environmental sex determination of the crustacean Daphnia magna.PLoS genetics · 2017Article
- CRISPR/Cas-mediated knock-in via non-homologous end-joining in the crustacean Daphnia magna.PloS one · 2017Article
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Authors and funding
4 authors.
Funding
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Abstract
Transcription activator-like effector nucleases (TALENs) are versatile tools that enable the insertion of DNA into different organisms. Here, we confirmed TALEN-mediated knock-in via non-homologous end joining in the crustacean Daphnia magna, a model organism for ecological and toxicological genomics. We tested two different TALENs, ey1 TALEN and ey2 TALEN, both of which target the eyeless locus. The donor DNA plasmid, harbouring the H2B-GFP reporter gene, was designed to contain both TALEN target sites and was co-injected with each TALEN mRNA into eggs. The ey1 TALEN and ey2 TALEN constructs both resulted in H2B-GFP expression in Daphnia with a germline transmission efficiency of 3%. Of the three transgenic animals generated, two had donor DNA at the targeted genomic site, which suggested concurrent cleavage of the injected plasmid DNA and genome DNA. The availability of such tools that are capable of targeted knock-in of foreign genes will be extremely useful for advancing the knowledge of gene function and contribute to an increased understanding of functional genomics in Daphnia.
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