ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2017
Adhesion of Escherichia coli under flow conditions reveals potential novel effects of FimH mutations.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Effect of human urinary microenvironment and fluid flow on antibiotic and phage therapy efficacy against uropathogenic Escherichia coli.Nature communications · 2026Article
- Genomic and phenotypic characterization of non-O157 Shiga toxin-producingMicrobiology spectrum · 2026Article
- Double agent: how Escherichia coli switches from commensal to pathogen in the urinary tract infection.Infection · 2026Review
- Lineage-specific type 1 fimbriae in hypervirulent ST23 Klebsiella pneumoniae target α3β1 integrin to promote intestinal epithelial invasion.BMC microbiology · 2026Article
- A flow cytometry method for quantitative measurement and molecular investigation of the adhesion of bacteria to yeast cells.Scientific reports · 2024Article
- Nature of the Interaction of Alpha-D-Mannose and Escherichia coli Bacteria, and Implications for its Regulatory Classification. A Delphi Panel European Consensus Based on Chemistry and Legal Evidence.Therapeutic innovation & regulatory science · 2023Article
- Insightful Improvement in the Design of Potent UropathogenicPharmaceutics · 2023Article
- Adhesion of Enteropathogenic, Enterotoxigenic, and Commensal Escherichia coli to the Major Zymogen Granule Membrane Glycoprotein 2.Applied and environmental microbiology · 2022Article
- Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems.Frontiers in cellular and infection microbiology · 2021Review
- Considerations on D-mannose Mechanism of Action and Consequent Classification of Marketed Healthcare Products.Frontiers in pharmacology · 2021Review
- CD48 and α7 Nicotinic Acetylcholine Receptor Synergistically Regulate FimH-Mediated Escherichia coli K1 Penetration and Neutrophil Transmigration Across Human Brain Microvascular Endothelial Cells.The Journal of infectious diseases · 2019Article
- Virulence factor-dependent basolateral invasion of choroid plexus epithelial cells by pathogenic Escherichia coli in vitro.FEMS microbiology letters · 2018Article
- Targeting Dynamical Binding Processes in the Design of Non-Antibiotic Anti-Adhesives by Molecular Simulation-The Example of FimH.Molecules (Basel, Switzerland) · 2018Review
- RINspector: a Cytoscape app for centrality analyses and DynaMine flexibility prediction.Bioinformatics (Oxford, England) · 2018Article
- Rational design strategies for FimH antagonists: new drugs on the horizon for urinary tract infection and Crohn's disease.Expert opinion on drug discovery · 2017Review
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
FimH-mediated adhesion of Escherichia coli to bladder epithelium is a prerequisite for urinary tract infections. FimH is also essential for blood-borne bacterial dissemination, but the mechanisms are poorly understood. The purpose of this study was to assess the influence of different FimH mutations on bacterial adhesion using a novel adhesion assay, which models the physiological flow conditions bacteria are exposed to. We introduced 12 different point mutations in the mannose binding pocket of FimH in an E. coli strain expressing type 1 fimbriae only (MSC95-FimH). We compared the bacterial adhesion of each mutant across several commonly used adhesion assays, including agglutination of yeast, adhesion to mono- and tri-mannosylated substrates, and static adhesion to bladder epithelial and endothelial cells. We performed a comparison of these assays to a novel method that we developed to study bacterial adhesion to mammalian cells under flow conditions. We showed that E. coli MSC95-FimH adheres more efficiently to microvascular endothelium than to bladder epithelium, and that only endothelium supports adhesion at physiological shear stress. The results confirmed that mannose binding pocket mutations abrogated adhesion. We demonstrated that FimH residues E50 and T53 are crucial for adhesion under flow conditions. The coating of endothelial cells on biochips and modelling of physiological flow conditions enabled us to identify FimH residues crucial for adhesion. These results provide novel insights into screening methods to determine the effect of FimH mutants and potentially FimH antagonists.
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