Evidence map›Paper›PMID 27816596›Full record

ArticleBiosensors & bioelectronics2017

Rapid capture and labeling of cells on single domain antibodies-functionalized flow cell.

Guan-Yu Chen, Zeyang Li, Joao N Duarte, Alexandre Esteban, Ross W Cheloha, Christopher S Theile, Gerald R Fink, Hidde L Ploegh

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biosensors & bioelectronics, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Current Conjugation Methods for Immunosensors.Nanomaterials (Basel, Switzerland) · 2018
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Guan-Yu ChenWhitehead Institute for Biomedical Research, Cambridge, MA, USA; Institute of Biomedical Engineering, National Chiao Tung University, Hsinchu 30010, Taiwan; Department of Biological Science and Technology, National Chiao Tung University, Hsinchu 30010, Taiwan.
Zeyang LiWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Joao N DuarteWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Alexandre EstebanWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Ross W ChelohaWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Christopher S TheileWhitehead Institute for Biomedical Research, Cambridge, MA, USA.
Gerald R FinkWhitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA.
Hidde L PloeghWhitehead Institute for Biomedical Research, Cambridge, MA, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA, USA. Electronic address: ploegh@wi.mit.edu.

Funding

REGULATION OF HISTIDINE BIOSYNTHESIS IN YEASTR01GM035010 · NIGMS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI FINK, GERALD R · 1985 to 2016
$9.9M
A new strategy to disrupt protein-protein interactions in eukaryotic cells.DP1GM106409 · NIGMS · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI PLOEGH, HIDDE L. · 2012 to 2016
$4.8M
Sortase-mediated protein engineering for the study of host-pathogen interactionsR01AI087879 · NIAID · WHITEHEAD INSTITUTE FOR BIOMEDICAL RES · PI PLOEGH, HIDDE L. · 2010 to 2019
$4.5M
NIAID NIH HHS R01 AI087879NIGMS NIH HHS DP1 GM106409NIGMS NIH HHS R01 GM035010
6 · The paper itself

Abstract

Current techniques to characterize leukocyte subgroups in blood require long sample preparation times and sizable sample volumes. A simplified method for leukocyte characterization using smaller blood volumes would thus be useful in diagnostic settings. Here we describe a flow system comprised of two functionalized graphene oxide (GO) surfaces that allow the capture of distinct leukocyte populations from small volumes blood using camelid single-domain antibodyfragments (VHHs) as capture agents. We used site-specifically labeled leukocytes to detect and identify cells exposed to fungal challenge. Combining the chemical and optical properties of GO with the versatility of the VHH scaffold in the context of a flow system provides a quick and efficient method for the capture and characterization of functional leukocytes.

Indexed as

AminoacyltransferasesAnimalsAntibodies, ImmobilizedBacterial ProteinsCandida albicansCandidiasisCell SeparationCysteine EndopeptidasesFemaleFlow CytometryGraphiteLeukocytesMice, Inbred C57BLMice, TransgenicNanostructuresSingle-Domain AntibodiesAminoacyltransferasesAntibodies, ImmobilizedBacterial ProteinsCysteine EndopeptidasesGraphiteSingle-Domain Antibodiessortase ACell detectionEnzymatic labelingGraphene oxideLeukocytesSingle domain antibodySortase

Identifiers

PMID27816596
PMCPMC5804343

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.