Evidence map›Paper›PMID 27816019›Full record

ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2016

Molecular detection of human parechovirus in under-Five-Year-Old Children with gastroenteritis.

Massimiliano Bergallo, Ilaria Galliano, Paola Montanari, Martina Rosa Brusin, Clara Gabiano

Open access · greenAbstract read
In one paragraph

Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
1.1field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 11 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 2 institutions in 1 country.

Massimiliano BergalloDepartment of Public Health and Pediatric Sciences, Citoimmunodiagnostics Laboratory, University of Turin, Medical School, 10136 Turin, Italy; SC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy. Electronic address: Massimiliano.bergallo@unito.it.
Ilaria GallianoDepartment of Public Health and Pediatric Sciences, Citoimmunodiagnostics Laboratory, University of Turin, Medical School, 10136 Turin, Italy; SC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy.
Paola MontanariDepartment of Public Health and Pediatric Sciences, Citoimmunodiagnostics Laboratory, University of Turin, Medical School, 10136 Turin, Italy; SC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy.
Martina Rosa BrusinSC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy.
Clara GabianoSC. Pediatric U Regina Margherita Hospital, Piazza Polonia 94, 10126 Turin, Italy.
Ospedale Regina Margherita · ITUniversity of Turin · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCurrently, RT-PCR is used widely and considered to be a convenient, useful, and powerful method for molecular diagnosis, to detect pathogens from clinical specimens.

objectivesIn this work we describe the development of an in-house Real-time Taqman PCR assay for quantification of HPeV in stool specimens. STUDY DESIGNS: A total of 137 fecal specimens previously screened for rotavirus and adenovirus were tested for HPeV virus.

resultsA total of 11 out of 137 (8%) episodes of acute gastroenteritis were associated with HPeV genomic detection with median viral load 14678±28927 genomes/mg fecal specimens. There was no significant difference in the detection rate between male and female (54.5% (6/11) vs. 45.5% (5/11). Among the 11 HPeV-positive cases, 2 were also positive for other viral pathogens, including rotavirus (n=2).

conclusionIn conclusion, the development of a laboratory designed Real Time PCR TaqMan assay for quantitative detection of HPeV and the optimization and standardization of this assay using stool of children with acute gastroenteritis are described.

Indexed as

Child, PreschoolFecesFemaleGastroenteritisHumansInfantInfant, NewbornMaleParechovirusPicornaviridae InfectionsReal-Time Polymerase Chain ReactionViral Loadco-infectionGastroenteritisParechovirusReal time-PCRRNA extraction

Identifiers

PMID27816019
PMCPMC7106367
OpenAlexW2546101817

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.