Evidence map›Paper›PMID 27815750›Full record

ArticleVirus genes2017

A sensitive duplex nanoparticle-assisted PCR assay for identifying porcine epidemic diarrhea virus and porcine transmissible gastroenteritis virus from clinical specimens.

Yu Zhu, Lin Liang, Yakun Luo, Guihua Wang, Chunren Wang, Yudong Cui, Xia Ai, Shangjin Cui

Open access · bronzeAbstract read
In one paragraph

Article in Virus genes, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
0.8field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 22 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Yu ZhuCollege of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing, 163319, China.
Lin LiangInstitute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Yuanmingyuan West Road 2, Hai Dian District, Beijing, 100193, China.
Yakun LuoInstitute of Animal Science (IAS), Chinese Academy of Agricultural Sciences (CAAS), Yuanmingyuan West Road 2, Hai Dian District, Beijing, 100193, China.
Guihua WangAnimal Medical Center DBN Technology Group, Beijing, 100195, China.
Chunren WangCollege of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing, 163319, China.
Yudong CuiCollege of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing, 163319, China.
Xia AiCollege of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, 300384, China. aixialucky@163.com.
Shangjin CuiCollege of Animal Science and Technology, HLJ August First Land Reclamation University, Daqing, 163319, China. cuishangjin@caas.cn.
Chinese Academy of Agricultural Sciences · CNHeilongjiang Academy of Land Reclamation Sciences · CNTianjin Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, a novel duplex nanoparticle-assisted polymerase chain reaction (nanoPCR) assay was developed to detect porcine epidemic diarrhea virus (PEDV) and porcine transmissible gastroenteritis virus (TGEV). Two pairs of primers were designed based on the conserved region within the N gene of PEDV and TGEV. In a screening of 114 clinical samples from four provinces in China for PEDV and TGEV, 48.2 and 3.5 % of the samples, respectively, tested positive. Under optimized conditions, the duplex nanoPCR assay had a detection limit of 7.6 × 10

Indexed as

NanoparticlesPolymerase Chain ReactionAnimalsPorcine epidemic diarrhea virusSensitivity and SpecificitySwineSwine DiseasesTransmissible gastroenteritis virusClinical diagnosisDuplex nanoPCRN genePEDVTGEV

Identifiers

PMID27815750
PMCPMC7089489
OpenAlexW2548653702

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.