ArticleThe Journal of biological chemistry2016
Plasma Membrane Origin of the Steroidogenic Pool of Cholesterol Used in Hormone-induced Acute Steroid Formation in Leydig Cells.
Article in The Journal of biological chemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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Who cites it
30 citing papers in PubMed, 53 citations in OpenAlex.
- Cholesterol at the Center of Alzheimer's Disease: A Unifying Hypothesis on the Pathogenic Mechanism.Molecules (Basel, Switzerland) · 2026Review
- Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2.iScience · 2026Article
- Review
- The effects of bisphenol A and its analogs on steroidogenesis in MA-10 Leydig cells and KGN granulosa cells†.Biology of reproduction · 2025Article
- Long-term dietary restriction changes lipid homeostasis and consequently impairs testosterone production in agedFrontiers in nutrition · 2025Article
- Maistero-2, a Novel Probe for Sterols: Application for Visualizing Cellular Cholesterol.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Lipid metabolism in the adrenal gland.Frontiers in endocrinology · 2025Review
- MicroRNA regulation of adrenal glucocorticoid and androgen biosynthesis.Vitamins and hormones · 2024Article
- HIV-1 Gag targeting to the plasma membrane reorganizes sphingomyelin-rich and cholesterol-rich lipid domains.Nature communications · 2023Article
- Delivery of low-density lipoprotein from endocytic carriers to mitochondria supports steroidogenesis.Nature cell biology · 2023Article
- Functional metabolite reserves and lipid homeostasis revealed by the MA-10 Leydig cell metabolome.PNAS nexus · 2022Article
- A novel sterol-binding protein reveals heterogeneous cholesterol distribution in neurite outgrowth and in late endosomes/lysosomes.Cellular and molecular life sciences : CMLS · 2022Article
- MEHP induces alteration of mitochondrial function and inhibition of steroid biosynthesis in MA-10 mouse tumor Leydig cells.Toxicology · 2021Article
- Distinctive functioning of STARD1 in the fetal Leydig cells compared to adult Leydig and adrenal cells. Impact of Hedgehog signaling via the primary cilium.Molecular and cellular endocrinology · 2021Article
- Dynamic Remodeling of Membranes and Their Lipids during Acute Hormone-Induced Steroidogenesis in MA-10 Mouse Leydig Tumor Cells.International journal of molecular sciences · 2021Article
- Sex steroids and steroidogenesis-related genes in the sea cucumber, Holothuria scabra and their potential role in gonad maturation.Scientific reports · 2021Article
- Sex Hormones and Lung Inflammation.Advances in experimental medicine and biology · 2021Article
- Disruption of membrane cholesterol organization impairs the activity of PIEZO1 channel clusters.The Journal of general physiology · 2020Article
- Identification of Sec23ip, Part of 14-3-3γ Protein Network, as a Regulator of Acute Steroidogenesis in MA-10 Leydig Cells.Endocrinology · 2020Article
- Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Hormone-sensitive acute steroid biosynthesis requires trafficking of cholesterol from intracellular sources to the inner mitochondrial membrane. The precise location of the intracellular cholesterol and its transport mechanism are uncertain. Perfringolysin O, produced by Clostridium perfringens, binds cholesterol. Its fourth domain (D4) retains cholesterol-binding properties but not cytotoxicity. We transfected steroidogenic MA-10 cells of mouse Leydig cell tumors with the mCherry-D4 plasmid. Tagged D4 with fluorescent proteins enabled us to track cholesterol. The staining was primarily localized to the inner leaflet of the plasma membrane and was partially released upon treatment with dibutyryl-cAMP (Bt
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.