ArticleThe EMBO journal2016
Inactivation of the type I interferon pathway reveals long double-stranded RNA-mediated RNA interference in mammalian cells.
Article in The EMBO journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 75 papers, 1 of them a synthesis that pooled it.
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Who cites it
75 citing papers in PubMed, 1 synthesis or guideline pooled it, 109 citations in OpenAlex.
- Pooled it
- Tuning intracellular immunity by Nodamura virus B2 protein enhances self-amplifying RNA activity.Nature communications · 2026Article
- Identification of Partitivirus-like RdRPs in theViruses · 2026Article
- Pluripotency Factors Modulate Interferon Signaling in Embryonic Stem Cells.bioRxiv : the preprint server for biology · 2026Article
- Induction of antiviral RNA interference by avian coronavirus IBV in PBMCs-Mφ.Cell communication and signaling : CCS · 2026Article
- Characterization of RNA interference in the cnidarian Nematostella vectensis reveals partial target silencing but lack of small RNA amplification.PLoS biology · 2026Article
- Host-targeted oral avian vaccine virus demonstrates broad antiviral activity and safety in patients.Frontiers in immunology · 2026Review
- Bat Dicer antiviral role and subcellular localization differ upon alphavirus infection in two distinct species.PLoS pathogens · 2025Article
- Nora virus proliferates in dividing intestinal stem cells and sensitizes flies to intestinal infection and oxidative stress.bioRxiv : the preprint server for biology · 2025Article
- Enhanced RNAi does not provide efficient innate antiviral immunity in mice.Nucleic acids research · 2025Article
- Investigating the Evolution of Drosophila STING-Dependent Antiviral Innate Immunity by Multispecies Comparison of 2'3'-cGAMP Responses.Molecular biology and evolution · 2024Article
- The helicase domain of human Dicer prevents RNAi-independent activation of antiviral and inflammatory pathways.The EMBO journal · 2024Article
- Invasion by exogenous RNA: cellular defense strategies and implications for RNA inference.Marine life science & technology · 2023Review
- Exogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context.Cellular & molecular biology letters · 2023Article
- Differential regulation of ATP hydrolysis of RIG-I-like receptors by transactivation response RNA-binding protein.Bioscience reports · 2023Article
- Ancestral protein reconstruction reveals evolutionary events governing variation in Dicer helicase function.eLife · 2023Article
- Mammalian viral suppressors of RNA interference.Trends in biochemical sciences · 2022Review
- STUB1 regulates antiviral RNAi through inducing ubiquitination and degradation of Dicer and AGO2 in mammals.Virologica Sinica · 2022Article
- Mouse circulating extracellular vesicles contain virus-derived siRNAs active in antiviral immunity.The EMBO journal · 2022Article
- SARS-CoV-2 RNAs are processed into 22-nt vsRNAs in Vero cells.Frontiers in immunology · 2022Article
15 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
RNA interference (RNAi) elicited by long double-stranded (ds) or base-paired viral RNA constitutes the major mechanism of antiviral defence in plants and invertebrates. In contrast, it is controversial whether it acts in chordates. Rather, in vertebrates, viral RNAs induce a distinct defence system known as the interferon (IFN) response. Here, we tested the possibility that the IFN response masks or inhibits antiviral RNAi in mammalian cells. Consistent with that notion, we find that sequence-specific gene silencing can be triggered by long dsRNAs in differentiated mouse cells rendered deficient in components of the IFN pathway. This unveiled response is dependent on the canonical RNAi machinery and is lost upon treatment of IFN-responsive cells with type I IFN Notably, transfection with long dsRNA specifically vaccinates IFN-deficient cells against infection with viruses bearing a homologous sequence. Thus, our data reveal that RNAi constitutes an ancient antiviral strategy conserved from plants to mammals that precedes but has not been superseded by vertebrate evolution of the IFN system.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.