ArticlePloS one2016
A One-Step Immunostaining Method to Visualize Rodent Muscle Fiber Type within a Single Specimen.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 64 citations in OpenAlex.
- Machine-learning classification of motor unit types in the adult mouse.The Journal of physiology · 2026Article
- Chronic cold induces some similar effects to endurance exercise on body morphometrics and skeletal muscle structure in rats.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2026Article
- The uncoordinated-5 homologue A is a key receptor in netrin-ligand-mediated fast-twitch myotube formation in male mice.Physiological reports · 2026Article
- Machine-Learning Classification of Motor Unit Types in the Adult Mouse.bioRxiv : the preprint server for biology · 2025Article
- R-spondin 3 is a myokine that mediates type I fiber determination during skeletal muscle regeneration.Molecular biology reports · 2025Article
- Fatigue Resistance and Mitochondrial Adaptations to Isometric Interval Training in Dystrophin-Deficient Muscle: Role of Contractile Load.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Article
- Optimizing Confocal Imaging Protocols for Muscle Fiber Typing in the Mouse Masseter Muscle.Bio-protocol · 2025Article
- Creation of knockin mice for the fluorescence protein based in vivo identification of skeletal myofiber types.Scientific reports · 2025Article
- Co-expression of MyHC-15 with other known isoforms in rat muscle spindles.European journal of histochemistry : EJH · 2025Article
- Non-muscle myosin IIC predominantly expressed in the slow-twitch skeletal muscles impedes age-related muscle weakness.PloS one · 2025Article
- Myokine BDNF highly expressed in Type I fibers inhibits the differentiation of myotubes into Type II fibers.Molecular biology reports · 2024Article
- Eicosapentaenoic acid increases proportion of type 1 muscle fibers through PPARδ and AMPK pathways in rats.iScience · 2024Article
- Article
- Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice.Scientific reports · 2024Article
- MuscleJ2: a rebuilding of MuscleJ with new features for high-content analysis of skeletal muscle immunofluorescence slides.Skeletal muscle · 2023Article
- Thyroid Hormone Regulates the Lipid Content of Muscle Fibers, Thus Affecting Physical Exercise Performance.International journal of molecular sciences · 2023Article
- Article
- Stable isotope-labeled carnitine reveals its rapid transport into muscle cells and acetylation during contraction.Heliyon · 2023Article
- The myonuclear domain in adult skeletal muscle fibres: past, present and future.The Journal of physiology · 2023Review
- Loss of FoxOs in muscle increases strength and mitochondrial function during aging.Journal of cachexia, sarcopenia and muscle · 2023Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, we present a quadruple immunostaining method for rapid muscle fiber typing of mice and rats using antibodies specific to the adult myosin heavy chain (MyHC) isoforms MyHC1, 2A, 2X, and 2B, which are common marker proteins of distinct muscle fiber types. We developed rat monoclonal antibodies specific to each MyHC isoform and conjugated these four antibodies to fluorophores with distinct excitation and emission wavelengths. By mixing the four types of conjugated antibodies, MyHC1, 2A, 2X, and 2B could be distinguished within a single specimen allowing for facile delineation of skeletal muscle fiber types. Furthermore, we could observe hybrid fibers expressing MyHC2X and MyHC2B together in single longitudinal muscle sections from mice and rats, that was not attained in previous techniques. This staining method is expected to be applied to study muscle fiber type transition in response to environmental factors, and to ultimately develop techniques to regulate animal muscle fiber types.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.