ArticleTraffic (Copenhagen, Denmark)2017
Cellular and viral peptides bind multiple sites on the N-terminal domain of clathrin.
Article in Traffic (Copenhagen, Denmark), 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 39 citations in OpenAlex.
- Inhibition of the Clathrin Terminal Domain-Amphiphysin Protein-Protein Interaction. Probing the Pitstop 2 Aromatic Moiety.ChemMedChem · 2025Article
- Using Large Language Model to Optimize Protein Purification: Insights from Protein Structure Literature Associated with Protein Data Bank.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Subtleties in Clathrin heavy chain binding boxes provide selectivity among adaptor proteins of budding yeast.Nature communications · 2024Article
- The Chemical Inhibitors of Endocytosis: From Mechanisms to Potential Clinical Applications.Cells · 2023Review
- Large-scale phage-based screening reveals extensive pan-viral mimicry of host short linear motifs.Nature communications · 2023Article
- An interaction between β'-COP and the ArfGAP, Glo3, maintains post-Golgi cargo recycling.The Journal of cell biology · 2023Article
- Identification of a novel interaction site between the large hepatitis delta antigen and clathrin that regulates the assembly of genotype III hepatitis delta virus.Virology journal · 2022Article
- Discrete GPCR-triggered endocytic modes enable β-arrestins to flexibly regulate cell signaling.eLife · 2022Article
- Multi-modal adaptor-clathrin contacts drive coated vesicle assembly.The EMBO journal · 2021Article
- Role of Clathrin and Dynamin in Clathrin Mediated Endocytosis/Synaptic Vesicle Recycling and Implications in Neurological Diseases.Frontiers in cellular neuroscience · 2021Review
- Evolving models for assembling and shaping clathrin-coated pits.The Journal of cell biology · 2020Review
- Wbox2: A clathrin terminal domain-derived peptide inhibitor of clathrin-mediated endocytosis.The Journal of cell biology · 2020Article
- Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.Science advances · 2020Article
- The structures of natively assembled clathrin-coated vesicles.Science advances · 2020Article
- Clathrin's adaptor interaction sites are repurposed to stabilize microtubules during mitosis.The Journal of cell biology · 2020Article
- Cryo-EM of multiple cage architectures reveals a universal mode of clathrin self-assembly.Nature structural & molecular biology · 2019Article
- Extreme Fuzziness: Direct Interactions between Two IDPs.Biomolecules · 2019Review
- The lipid transfer protein Saposin B does not directly bind CD1d for lipid antigen loading.Wellcome open research · 2019Article
- Behaviour of intrinsically disordered proteins in protein-protein complexes with an emphasis on fuzziness.Cellular and molecular life sciences : CMLS · 2017Review
- Weak Molecular Interactions in Clathrin-Mediated Endocytosis.Frontiers in molecular biosciences · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 3 countries.
Funding
Abstract
Short peptide motifs in unstructured regions of clathrin-adaptor proteins recruit clathrin to membranes to facilitate post-Golgi membrane transport. Three consensus clathrin-binding peptide sequences have been identified and structural studies show that each binds distinct sites on the clathrin heavy chain N-terminal domain (NTD). A fourth binding site for adaptors on NTD has been functionally identified but not structurally characterised. We have solved high resolution structures of NTD bound to peptide motifs from the cellular clathrin adaptors β2 adaptin and amphiphysin plus a putative viral clathrin adaptor, hepatitis D virus large antigen (HDAg-L). Surprisingly, with each peptide we observe simultaneous peptide binding at multiple sites on NTD and viral peptides binding to the same sites as cellular peptides. Peptides containing clathrin-box motifs (CBMs) with the consensus sequence LΦxΦ[DE] bind at the 'arrestin box' on NTD, between β-propeller blades 4 and 5, which had previously been thought to bind a distinct consensus sequence. Further, we structurally define the fourth peptide binding site on NTD, which we term the Royle box. In vitro binding assays show that clathrin is more readily captured by cellular CBMs than by HDAg-L, and site-directed mutagenesis confirms that multiple binding sites on NTD contribute to efficient capture by CBM peptides.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.