ArticleCell stress & chaperones2017
Use of conditioned media is critical for studies of regulation in response to rapid heat shock.
Article in Cell stress & chaperones, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 24 citations in OpenAlex.
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- Transcriptional responses of cancer cells to heat shock-inducing stimuli involve amplification of robust HSF1 binding.Nature communications · 2023Article
- PRO-IP-seq tracks molecular modifications of engaged Pol II complexes at nucleotide resolution.Nature communications · 2023Article
- Adaptive preservation of orphan ribosomal proteins in chaperone-dispersed condensates.Nature cell biology · 2023Article
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- Transcriptional lockdown during acute proteotoxic stress.Trends in biochemical sciences · 2022Review
- KAS-seq: genome-wide sequencing of single-stranded DNA by NNature protocols · 2022Review
- Stress-induced transcriptional memory accelerates promoter-proximal pause release and decelerates termination over mitotic divisions.Molecular cell · 2021Article
- Acute perturbation strategies in interrogating RNA polymerase II elongation factor function in gene expression.Genes & development · 2021Article
- RNA aptamer capture of macromolecular complexes for mass spectrometry analysis.Nucleic acids research · 2020Article
- Native molecule sequencing by nano-ID reveals synthesis and stability of RNA isoforms.Genome research · 2020Article
- Analysis of RNA polymerase II ubiquitylation and proteasomal degradation.Methods (San Diego, Calif.) · 2019Article
- Nascent-protein ubiquitination is required for heat shock-induced gene downregulation in human cells.Nature structural & molecular biology · 2019Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Heat shock response (HSR) maintains and restores protein homeostasis when cells are exposed to proteotoxic heat stress. Heat shock (HS) triggers a rapid and robust change in genome-wide transcription, protein synthesis, and chaperone activity; and therefore, the HSR has been widely used as a model system in these studies. The conventional method of performing instantaneous HS in the laboratory uses heated fresh media to induce HSR when added to cells. However, addition of fresh media to cells may evoke additional cellular responses and signaling pathways. Here, we compared the change in global transcription profile when HS is performed with either heated fresh media or heated conditioned media. We found that the use of heated fresh media induces transcription of hundreds of genes that HS alone does not induce, and masks or partially masks HS-mediated downregulation of thousands of genes. The fresh-media-dependent upregulated genes encode ribosomal subunit proteins involved in translation and RNA processing factors. More importantly, fresh media also induce transcription of several heat shock protein genes (Hsps) in a heat shock factor 1 (HSF1)-independent manner. Thus, we conclude that a conventional method of HS with heated fresh media causes changes in transcription regulation that confound the actual change caused solely by elevated temperature of cells.
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