Evidence map›Paper›PMID 27812189›Full record

ArticlePloS one2016

Mesenchymal Stem/Stromal Cells under Stress Increase Osteosarcoma Migration and Apoptosis Resistance via Extracellular Vesicle Mediated Communication.

Krishna C Vallabhaneni, Meeves-Yoni Hassler, Anu Abraham, Jason Whitt, Yin-Yuan Mo, Azeddine Atfi, Radhika Pochampally

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.

0numbers the graph read from it
0cells of the map it votes in
53citing papers in PubMed
4.2field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

53 citing papers in PubMed, 85 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 2 institutions in 1 country.

Krishna C VallabhaneniCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Meeves-Yoni HasslerCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Anu AbrahamCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Jason WhittCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Yin-Yuan MoCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Azeddine AtfiCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
Radhika PochampallyCancer Institute, University of Mississippi Medical Center, Jackson, MS, United States of America.
University of Mississippi Medical Center · USJackson Memorial Hospital · US

Funding

Role of TGIF in Wnt-Induced Bone FormationR01AR059070 · NIAMS · UNIVERSITY OF MISSISSIPPI MED CTR · PI ATFI, AZEDDINE · 2010 to 2014
$1.7M
miRNA-mediated Stromal Cell Survival under Stress Supports Breast Cancer GrowthR01CA151851 · NCI · UNIVERSITY OF MISSISSIPPI MED CTR · PI POCHAMPALLY, RADHIKA R · 2011 to 2016
$1.5M
NCI NIH HHS R01 CA151851NIAMS NIH HHS R01 AR059070
6 · The paper itself

Abstract

Studies have shown that mesenchymal stem/stromal cells (MSCs) from bone marrow are involved in the growth and metastasis of solid tumors but the mechanism remains unclear in osteosarcoma (OS). Previous studies have raised the possibility that OS cells may receive support from associated MSCs in the nutrient deprived core of the tumors through the release of supportive macromolecules and growth factors either in vesicular or non-vesicular forms. In the present study, we used stressed mesenchymal stem cells (SD-MSCs), control MSCs and OS cells to examine the hypothesis that tumor-associated MSCs in nutrient deprived core provide pro-proliferative, anti-apoptotic, and metastatic support to nearby tumor cells. Assays to study of the effects of SD-MSC conditioned media revealed that OS cells maintained proliferation when compared to OS cells grown under serum-starved conditions alone. Furthermore, OS cells in MSCs and SD-MSC conditioned media were significantly resistant to apoptosis and an increased wound healing rate was observed in cells exposed to either conditioned media or EVs from MSCs and SD-MSCs. RT-PCR assays of OS cells incubated with extracellular vesicles (EVs) from SD-MSCs revealed microRNAs that could potentially target metabolism and metastasis associated genes as predicted by in silico algorithms, including monocarboxylate transporters, bone morphogenic receptor type 2, fibroblast growth factor 7, matrix metalloproteinase-1, and focal adhesion kinase-1. Changes in the expression levels of focal adhesion kinase, STK11 were confirmed by quantitative PCR assays. Together, these data indicate a tumor supportive role of MSCs in osteosarcoma growth that is strongly associated with the miRNA content of the EVs released from MSCs under conditions that mimic the nutrient deprived core of solid tumors.

Indexed as

ApoptosisCell CommunicationCell MovementOxidative StressCell Line, TumorCell ProliferationCell SurvivalCulture Media, ConditionedExtracellular VesiclesGene Expression Regulation, NeoplasticHumansIntercellular Signaling Peptides and ProteinsMesenchymal Stem CellsMicroRNAsOsteosarcomaTumor MicroenvironmentCulture Media, ConditionedIntercellular Signaling Peptides and ProteinsMicroRNAs

Identifiers

PMID27812189
PMCPMC5094708
OpenAlexW2546842071

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.