ArticleScientific reports2016
On-chip, multisite extracellular and intracellular recordings from primary cultured skeletal myotubes.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 38 citations in OpenAlex.
- Differentiated skeletal myotubes coupled with bimodal biosensing for dynamic functional evaluation of inflammatory myotube dysfunction.Microsystems & nanoengineering · 2026Article
- CASCADE: criticality avalanche spike cross-platform analysis detection engine, a multi-manufacturer MEA bash analysis pipeline.Bioinformatics (Oxford, England) · 2026Article
- Small Extracellular Vesicle Release Following Electrical Pulse Stimulation of C2C12 Myotubes: Effects on microRNA Cargo and Myoblast Migration and Differentiation.International journal of molecular sciences · 2026Article
- Toward continuous monitoring systems: emerging trends of on-chip sensors in organ-on-a-chip.Microsystems & nanoengineering · 2026Review
- Culture and Electrophysiological Analysis of Patient-Specific iPSCs Using Microelectrode Array Dishes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Advanced passive 3D bioelectronics: powerful tool for the cardiac electrophysiology investigation.Microsystems & nanoengineering · 2025Review
- Optimization of Application-Driven Development ofTissue engineering. Part B, Reviews · 2022Review
- Cardiotoxicity drug screening based on whole-panel intracellular recording.Biosensors & bioelectronics · 2022Article
- Review
- Ultrastructural Analysis of Neuroimplant-Parenchyma Interfaces Uncover Remarkable Neuroregeneration Along-With Barriers That Limit the Implant Electrophysiological Functions.Frontiers in neuroscience · 2021Article
- Assessing the Feasibility of DevelopingFrontiers in neuroscience · 2021Article
- Microelectrode array analysis of mouse uterine smooth muscle electrical activity†.Biology of reproduction · 2020Article
- Development of 3D neuromuscular bioactuators.APL bioengineering · 2020Article
- High-Aspect-Ratio Nanostructured Surfaces as Biological Metamaterials.Advanced materials (Deerfield Beach, Fla.) · 2020Review
- Opportunities and dilemmas ofRSC advances · 2019Review
- Interfacing Cells with Vertical Nanoscale Devices: Applications and Characterization.Annual review of analytical chemistry (Palo Alto, Calif.) · 2018Review
- Long-Term High-Density Extracellular Recordings Enable Studies of Muscle Cell Physiology.Frontiers in physiology · 2018Article
- Multisite Attenuated Intracellular Recordings by Extracellular Multielectrode Arrays, a Perspective.Frontiers in neuroscience · 2018Article
- Intracellular and Extracellular Recording of Spontaneous Action Potentials in Mammalian Neurons and Cardiac Cells with 3D Plasmonic Nanoelectrodes.Nano letters · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In contrast to the extensive use of microelectrode array (MEA) technology in electrophysiological studies of cultured neurons and cardiac muscles, the vast field of skeletal muscle research has yet to adopt the technology. Here we demonstrate an empowering MEA technology for high quality, multisite, long-term electrophysiological recordings from cultured skeletal myotubes. Individual rat skeletal myotubes cultured on micrometer sized gold mushroom-shaped microelectrode (gMμE) based MEA tightly engulf the gMμEs, forming a high seal resistance between the myotubes and the gMμEs. As a consequence, spontaneous action potentials generated by the contracting myotubes are recorded as extracellular field potentials with amplitudes of up to 10 mV for over 14 days. Application of a 10 ms, 0.5-0.9 V voltage pulse through the gMμEs electroporated the myotube membrane, and transiently converted the extracellular to intracellular recording mode for 10-30 min. In a fraction of the cultures stable attenuated intracellular recordings were spontaneously produced. In these cases or after electroporation, subthreshold spontaneous potentials were also recorded. The introduction of the gMμE-MEA as a simple-to-use, high-quality electrophysiological tool together with the progress made in the use of cultured human myotubes opens up new venues for basic and clinical skeletal muscle research, preclinical drug screening, and personalized medicine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.