ArticleJournal of experimental botany2016
Visualization of specific repetitive genomic sequences with fluorescent TALEs in Arabidopsis thaliana.
Article in Journal of experimental botany, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 35 citations in OpenAlex.
- Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo.Communications biology · 2023Article
- Visualizing the Nucleome Using the CRISPR-Cas9 System: From in vitro to in vivo.Biochemistry. Biokhimiia · 2023Review
- Review
- Plant 3D genomics: the exploration and application of chromatin organization.The New phytologist · 2021Review
- ANCHOR: A Technical Approach to Monitor Single-Copy Locus LocalizationFrontiers in plant science · 2021Article
- Subnuclear gene positioning through lamina association affects copper tolerance.Nature communications · 2020Article
- Tidying-up the plant nuclear space: domains, functions, and dynamics.Journal of experimental botany · 2020Review
- Decrosslinking enables visualization of RNA-guided endonuclease-in situ labeling signals for DNA sequences in plant tissues.Journal of experimental botany · 2020Article
- Application and prospects of CRISPR/Cas9-based methods to trace defined genomic sequences in living and fixed plant cells.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2020Review
- Kleptoprotein bioluminescence:Science advances · 2020Article
- Application of Aptamers Improves CRISPR-Based Live Imaging of Plant Telomeres.Frontiers in plant science · 2020Article
- HIGH STEROL ESTER 1 is a key factor in plant sterol homeostasis.Nature plants · 2019Article
- LSD1-LIKE1-Mediated H3K4me2 Demethylation Is Required for Homologous Recombination Repair.Plant physiology · 2019Article
- Fluorescence in situ hybridization in plants: recent developments and future applications.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2019Review
- RNA-guided endonuclease - in situ labelling (RGEN-ISL): a fast CRISPR/Cas9-based method to label genomic sequences in various species.The New phytologist · 2019Article
- Auxin decreases chromatin accessibility through the TIR1/AFBs auxin signaling pathway in proliferative cells.Scientific reports · 2018Article
- Live-cell CRISPR imaging in plants reveals dynamic telomere movements.The Plant journal : for cell and molecular biology · 2017Article
- Live imaging of H3K9 acetylation in plant cells.Scientific reports · 2017Article
- Visualization of aging-associated chromatin alterations with an engineered TALE system.Cell research · 2017Article
- A cautionary note on the use of chromosome conformation capture in plants.Plant methods · 2017Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live imaging of the dynamics of nuclear organization provides the opportunity to uncover the mechanisms responsible for four-dimensional genome architecture. Here, we describe the use of fluorescent protein (FP) fusions of transcription activator-like effectors (TALEs) to visualize endogenous genomic sequences in Arabidopsis thaliana. The ability to engineer sequence-specific TALEs permits the investigation of precise genomic sequences. We could detect TALE-FP signals associated with centromeric, telomeric, and rDNA repeats and the signal distribution was consistent with that observed by fluorescent in situ hybridization. TALE-FPs are advantageous because they permit the observation of intact tissues. We used our TALE-FP method to investigate the nuclei of several multicellular plant tissues including roots, hypocotyls, leaves, and flowers. Because TALE-FPs permit live-cell imaging, we successfully observed the temporal dynamics of centromeres and telomeres in plant organs. Fusing TALEs to multimeric FPs enhanced the signal intensity when observing telomeres. We found that the mobility of telomeres was different in sub-nuclear regions. Transgenic plants stably expressing TALE-FPs will provide new insights into chromatin organization and dynamics in multicellular organisms.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.