ArticleOpen biology2016
The Drosophila chromosomal protein Mst77F is processed to generate an essential component of mature sperm chromatin.
Article in Open biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 35 citations in OpenAlex.
- Rapid protamine evolution suppresses meiotic drive inScience advances · 2026Article
- The jewel wasp Nasonia vitripennis utilizes two single-copy, protamine-like sperm nuclear basic proteins.G3 (Bethesda, Md.) · 2026Article
- Selfish chromosomes exploit a germline checkpoint to eliminate competing gametes.Nature communications · 2026Article
- A comparison of spermatogenesis between flies and men-conserved processes of male gamete production.Human reproduction update · 2025Review
- Intrinsically weak sex chromosome drive through sequential asymmetric meiosis.Science advances · 2025Article
- Involvement of Histone Acetyltransferase 1 (HAT1) in the Spermatogenesis of Non-Condensed Nuclear Sperm in Chinese Mitten Crab, Eriocheir sinensis.Biochemical genetics · 2025Article
- Running the gauntlet: challenges to genome integrity in spermiogenesis.Nucleus (Austin, Tex.) · 2024Review
- New perspectives on the causes and consequences of male meiotic drive.Current opinion in genetics & development · 2023Review
- Histone removal in sperm protects paternal chromosomes from premature division at fertilization.Science (New York, N.Y.) · 2023Article
- Single-cell transcriptomics identifies new blood cell populations in Drosophila released at the onset of metamorphosis.Development (Cambridge, England) · 2023Article
- Nuclear elongation during spermiogenesis depends on physical linkage of nuclear pore complexes to bundled microtubules by Drosophila Mst27D.PLoS genetics · 2023Article
- Essential and recurrent roles for hairpin RNAs in silencing de novo sex chromosome conflict in Drosophila simulans.PLoS biology · 2023Article
- Broad phosphorylation mediated by testis-specific serine/threonine kinases contributes to spermiogenesis and male fertility.Nature communications · 2023Article
- Derepression of Y-linked multicopy protamine-like genes interferes with sperm nuclear compaction inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Article
- Single-cell transcriptome sequencing revealsProceedings. Biological sciences · 2023Article
- A rapidly evolving single copy histone H1 variant is associated with male fertility in a parasitoid wasp.Frontiers in cell and developmental biology · 2023Article
- A putative de novo evolved gene required for spermatid chromatin condensation in Drosophila melanogaster.PLoS genetics · 2021Article
- salto/CG13164 is required for sperm head morphogenesis in Drosophila.Molecular biology of the cell · 2019Article
- Essential Role of Histone Replacement and Modifications in Male Fertility.Frontiers in genetics · 2019Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In most animals, the bulk of sperm DNA is packaged with sperm nuclear basic proteins (SNBPs), a diverse group of highly basic chromosomal proteins notably comprising mammalian protamines. The replacement of histones with SNBPs during spermiogenesis allows sperm DNA to reach an extreme level of compaction, but little is known about how SNBPs actually function in vivo Mst77F is a Drosophila SNBP with unique DNA condensation properties in vitro, but its role during spermiogenesis remains unclear. Here, we show that Mst77F is required for the compaction of sperm DNA and the production of mature sperm, through its cooperation with protamine-like proteins Mst35Ba/b. We demonstrate that Mst77F is incorporated in spermatid chromatin as a precursor protein, which is subsequently processed through the proteolysis of its N-terminus. The cleavage of Mst77F is very similar to the processing of protamine P2 during human spermiogenesis and notably leaves the cysteine residues in the mature protein intact, suggesting that they participate in the formation of disulfide cross-links. Despite the rapid evolution of SNBPs, sperm chromatin condensation thus involves remarkably convergent mechanisms in distantly related animals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.