ArticleMutation research
Transposon-mediated activation of the Escherichia coli glpFK operon is inhibited by specific DNA-binding proteins: Implications for stress-induced transposition events.
Article in Mutation research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 13 citations in OpenAlex.
- Transcriptional mechanism by which IS5 activates the fucAO operon in Escherichia coli.Nucleic acids research · 2025Article
- Effects of Global and Specific DNA-Binding Proteins on Transcriptional Regulation of theInternational journal of molecular sciences · 2022Article
- Insertion Sequence (IS) Element-Mediated Activating Mutations of the Cryptic Aromatic β-Glucoside Utilization (International journal of molecular sciences · 2022Article
- Frequent Transposition of Multiple Insertion Sequences inFrontiers in microbiology · 2021Article
- Competition for nutritional resources masks the true frequency of bacterial mutants.BMC biology · 2020Article
- Intensive targeting of regulatory competence genes by transposable elements in streptococci.Molecular genetics and genomics : MGG · 2019Article
- Environment-directed activation of the Escherichia coliflhDC operon by transposons.Microbiology (Reading, England) · 2017Article
- Transposon-mediated directed mutation in bacteria and eukaryotes.Frontiers in bioscience (Landmark edition) · 2017Review
- Hopping into a hot seat: Role of DNA structural features on IS5-mediated gene activation and inactivation under stress.PloS one · 2017Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Escherichia coli cells deleted for the cyclic AMP (cAMP) receptor protein (Crp) gene (Δcrp) cannot utilize glycerol because cAMP-Crp is a required activator of the glycerol utilization operon, glpFK. We have previously shown that a transposon, Insertion Sequence 5 (IS5), can insert into the upstream regulatory region of the operon to activate the glpFK promoter and enable glycerol utilization. GlpR, which represses glpFK transcription, binds to the glpFK upstream region near the site of IS5 insertion and inhibits insertion. By adding cAMP to the culture medium in ΔcyaA cells, we here show that the cAMP-Crp complex, which also binds to the glpFK upstream regulatory region, inhibits IS5 hopping into the activating site. Control experiments showed that the frequencies of mutations in response to cAMP were independent of parental cell growth rate and the selection procedure. These findings led to the prediction that glpFK-activating IS5 insertions can also occur in wild-type (Crp
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