ArticleMetabolic engineering2017
Metabolic engineering of Saccharomyces cerevisiae for de novo production of dihydrochalcones with known antioxidant, antidiabetic, and sweet tasting properties.
Article in Metabolic engineering, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 116 citations in OpenAlex.
- Dihydrochalcones: Methods of Acquisition and Pharmacological Properties-A First Systematic Review.Molecules (Basel, Switzerland) · 2019Pooled it
- Study on biosynthesis pathway and accumulation mechanism of the dihydrochalcones inHorticulture research · 2026Article
- Tracing origin and cultivation practice of Lithocarpus litseifolius via multi-data fusion and machine learning approaches.NPJ science of food · 2026Article
- Multidimensional Profiling of Chinese Sweet TeaFoods (Basel, Switzerland) · 2025Article
- Identification of a hydroxycinnamoyl-CoA double bond reductase (HDR) affirms multiple pathways for dihydrochalcone formation in apple.Plant biotechnology journal · 2025Article
- Effect of Sunlight-Induced Isomerisation on the Biotransformation of 4'-Hydroxychalcones byInternational journal of molecular sciences · 2025Article
- Reinforced NADPH regeneration in engineered Saccharomyces cerevisiae enhances rosmarinic acid production.Archives of microbiology · 2025Article
- Biosynthesis and metabolic engineering of natural sweeteners.AMB Express · 2025Review
- Engineering Saccharomyces cerevisiae for medical applications.Microbial cell factories · 2025Review
- Naringenin chalcone carbon double-bond reductases mediate dihydrochalcone biosynthesis in apple leaves.Plant physiology · 2024Article
- New insights into the roles of fungi and bacteria in the development of medicinal plant.Journal of advanced research · 2024Review
- Optimizing Trilobatin Production via Screening and Modification of Glycosyltransferases.Molecules (Basel, Switzerland) · 2024Article
- Xylose and shikimate transporters facilitates microbial consortium as a chassis for benzylisoquinoline alkaloid production.Nature communications · 2023Article
- The catalytic role of glutathione transferases in heterologous anthocyanin biosynthesis.Nature catalysis · 2023Article
- Naringenin Prevents Oxidative Stress and Inflammation in LPS-Induced Liver Injury through the Regulation of LncRNA-mRNA in Male Mice.Molecules (Basel, Switzerland) · 2022Article
- AACS synthetic biology · 2022Article
- Metabolic engineering of Yarrowia lipolytica for scutellarin production.Synthetic and systems biotechnology · 2022Article
- First purified recombinant CYP75B including transmembrane helix with unexpected high substrate specificity to (2R)-naringenin.Scientific reports · 2022Article
- Article
- Molecular and Enzymatic Characterization of Flavonoid 3'-Hydroxylase ofPlants (Basel, Switzerland) · 2021Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dihydrochalcones are plant secondary metabolites comprising molecules of significant commercial interest as antioxidants, antidiabetics, or sweeteners. To date, their heterologous biosynthesis in microorganisms has been achieved only by precursor feeding or as minor by-products in strains engineered for flavonoid production. Here, the native ScTSC13 was overexpressed in Saccharomyces cerevisiae to increase its side activity in reducing p-coumaroyl-CoA to p-dihydrocoumaroyl-CoA. De novo production of phloretin, the first committed dihydrochalcone, was achieved by co-expression of additional relevant pathway enzymes. Naringenin, a major by-product of the initial pathway, was practically eliminated by using a chalcone synthase from barley with unexpected substrate specificity. By further extension of the pathway from phloretin with decorating enzymes with known specificities for dihydrochalcones, and by exploiting substrate flexibility of enzymes involved in flavonoid biosynthesis, de novo production of the antioxidant molecule nothofagin, the antidiabetic molecule phlorizin, the sweet molecule naringin dihydrochalcone, and 3-hydroxyphloretin was achieved.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.