ArticleVeterinary parasitology2016
Application of a new PCR-RFLP panel suggests a restricted population structure for Eimeria tenella in UK and Irish chickens.
Article in Veterinary parasitology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
6 citing papers in PubMed, 11 citations in OpenAlex.
- Prevalence of Avian coccidiosis in India: a review.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2024Review
- Prevalence and Risk Factors ofPathogens (Basel, Switzerland) · 2024Article
- Benefits and limitations of a new genome-based PCR-RFLP genotyping assay (GB-RFLP): A SNP-based detection method for identification of species in extremely young adaptive radiations.Ecology and evolution · 2022Article
- ExploringGenes · 2020Review
- Microsatellite Analysis of Geographically Close Isolates ofFrontiers in veterinary science · 2019Article
- Diagnosis and control of chicken coccidiosis: a recent update.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2018Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Eimeria species cause coccidiosis, most notably in chickens where the global cost exceeds US$3 billion every year. Understanding variation in Eimeria population structure and genetic diversity contributes valuable information that can be used to minimise the impact of drug resistance and develop new, cost-effective anticoccidial vaccines. Little knowledge is currently available on the epidemiology of Eimeria species and strains in different regions, or under different chicken production systems. Recently, 244 Eimeria tenella isolates collected from countries in Africa and Asia were genotyped using a Sequenom single nucleotide polymorphism (SNP) tool, revealing significant variation in haplotype diversity and population structure, with a marked North/South regional divide. To expand studies on genetic polymorphism to larger numbers of E. tenella populations in other geographic regions a cheaper and more accessible technique, such as polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP), is desirable. We have converted a subset of SNP markers for use as PCR-RFLPs and re-analysed the original 244 isolates with the PCR-RFLPs to assess their utility. In addition, application of the PCR-RFLP to E. tenella samples collected from UK and Irish broiler chickens revealed a tightly restricted haplotype diversity. Just two of the PCR-RFLPs accounted for all of the polymorphism detected in the UK and Irish parasite populations, but analysis of the full dataset revealed different informative markers in different regions, supporting validity of the PCR-RFLP panel. The tools described here provide an accessible and cost-effective method that can be used to enhance understanding of E. tenella genetic diversity and population structure.
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Registered trials
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