Evidence map›Paper›PMID 27809904›Full record

ArticleBMC structural biology2016

Controlled dehydration improves the diffraction quality of two RNA crystals.

HaJeung Park, Tuan Tran, Jun Hyuck Lee, Hyun Park, Matthew D Disney

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC structural biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Functional Water Networks in Fully Hydrated Photosystem II.Journal of the American Chemical Society · 2022
    Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

HaJeung ParkX-ray Core Facility, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA. hajpark@scripps.edu.ORCID 0000-0003-4898-7919
Tuan TranDepartment of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA.
Jun Hyuck LeeUnit of Polar Genomics, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
Hyun ParkUnit of Polar Genomics, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
Matthew D DisneyDepartment of Chemistry, The Scripps Research Institute, Scripps Florida, 130 Scripps Way, Jupiter, FL, 33458, USA. disney@scripps.edu.

Funding

Sequence-based Design of Small Molecules Targeting RNAR01GM097455 · NIGMS · SCRIPPS FLORIDA · PI DISNEY, MATTHEW D · 2012 to 2019
$4.6M
NIGMS NIH HHS R01 GM097455
6 · The paper itself

Abstract

backgroundPost-crystallization dehydration methods, applying either vapor diffusion or humidity control devices, have been widely used to improve the diffraction quality of protein crystals. Despite the fact that RNA crystals tend to diffract poorly, there is a dearth of reports on the application of dehydration methods to improve the diffraction quality of RNA crystals.

resultsWe use dehydration techniques with a Free Mounting System (FMS, a humidity control device) to recover the poor diffraction quality of RNA crystals. These approaches were applied to RNA constructs that model various RNA-mediated repeat expansion disorders.

conclusionThe method we describe herein could serve as a general tool to improve diffraction quality of RNA crystals to facilitate structure determinations.

Indexed as

Base SequenceCrystallizationDesiccationNucleic Acid ConformationRNAX-Ray DiffractionRNACrystal dehydrationFree mounting systemPost-crystallization modificationRepeat expansionRNA

Identifiers

PMID27809904
PMCPMC5093936

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.