ArticleMalaria journal2016
Serological markers to measure recent changes in malaria at population level in Cambodia.
Article in Malaria journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers, 1 of them a synthesis that pooled it.
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Who cites it
42 citing papers in PubMed, 1 synthesis or guideline pooled it, 60 citations in OpenAlex.
- Pooled it
- Assessment of antibody responses to Anopheles SG6-P1 and Aedes N-term 34 kDa salivary peptides: a randomised human-challenge trial of controlled exposures to vector bites.BMC medicine · 2026Trial
- Trial
- Geographical patterns of malaria transmission based on serological markers for falciparum and vivax malaria in Ratanakiri, Cambodia.Malaria journal · 2016Trial
- Comparative evaluation of four immunoassays for Plasmodium vivax serological detection using recombinant PvMSP1-42 antigen.PLoS neglected tropical diseases · 2026Article
- Serological surveillance for malaria elimination in Southeast Asia: a scoping review.Malaria journal · 2026Article
- Pf41 serological profiling reveals potential malaria exposure in low transmission setting in the Menoreh Hills, Central Java, Indonesia.BMC microbiology · 2026Article
- Optimizing age-structured sampling for estimating the seroconversion rate in malaria seroepidemiology: a simulation study.Scientific reports · 2026Article
- Naturally acquired antibodies toFrontiers in immunology · 2026Article
- Serology reveals comparable patterns in the transmission intensities ofFrontiers in cellular and infection microbiology · 2025Article
- Copy Number Variations of Plasmodium vivax DBP1, EBP/DBP2, and RBP2b in Ethiopians Who Are Duffy Positive and Duffy Negative.The Journal of infectious diseases · 2024Article
- Analytical approaches for antimalarial antibody responses to confirm historical and recent malaria transmission: an example from the Philippines.The Lancet regional health. Western Pacific · 2023Article
- Using Serological Markers for the Surveillance ofPathogens (Basel, Switzerland) · 2023Article
- Seroprevalence and Risk Factors of Lyme Borreliosis in The Netherlands: A Population-Based Cross-Sectional Study.Microorganisms · 2023Article
- Serology reveals micro-differences inFrontiers in parasitology · 2023Article
- Etramp5 as a useful serological marker in children to assess the immediate effects of mass drug campaigns for malaria.BMC infectious diseases · 2022Article
- Quantification of the dynamics of antibody response to malaria to inform sero-surveillance in pregnant women.Malaria journal · 2022Article
- Cross-sectional study of asymptomatic malaria and seroepidemiological surveillance of seven districts in Gia Lai province, Vietnam.Malaria journal · 2022Article
- Predictive markers of transmission in areas with different malaria endemicity in north-eastern Tanzania based on seroprevalence of antibodies against Plasmodium falciparum.BMC research notes · 2021Article
- Community-based molecular and serological surveillance of subclinical malaria in Myanmar.BMC medicine · 2021Article
Corrections and comments
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Authors and funding
12 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSerological markers for exposure to different Plasmodium species have recently been used in multiplex immunoassays based on the Luminex technology. However, interpretation of the assay results requires consideration of the half-life of specific antibodies against these markers. Therefore, the aim of the present study was to document the half-life of malaria specific serological makers, as well as assessing the sensitivity of these markers to pick up recent changes in malaria exposure.
methodsA recently developed multiplex immunoassay was used to measure the intensity of antibody (Ab) responses against 19 different Plasmodium specific antigens, covering different human malaria parasites and two vector saliva antigens. Therefore, 8439 blood samples from five cross-sectional surveys in Ratanakiri, Cambodia, were analysed. These involve a random selection from two selected surveys, and an additional set of blood samples of individuals that were randomly re-sampled three, four or five times. A generalized estimating equation model and linear regression models were fitted on log transformed antibody intensity data.
resultsResults showed that most (17/21) Ab-responses are higher in PCR positive than PCR negative individuals. Furthermore, these antibody-responses follow the same upward trend within each age group. Estimation of the half-lives showed differences between serological markers that reflect short- (seasonal) and long-term (year round) transmission trends. Ab levels declined significantly together with a decrease of PCR prevalence in a group of malaria endemic villages.
conclusionFor Plasmodium falciparum, antibodies against LSA3.RE, GLURP and Pf.GLURP.R2 are most likely to be a reflexion of recent (range from 6 to 8 months) exposure in the Mekong Subregion. PvEBP is the only Plasmodium vivax Ag responding reasonably well, in spite of an estimated Ab half-life of more than 1 year. The use of Ab intensity data rather dichotomizing the continuous Ab-titre data (positive vs negative) will lead to an improved approach for serological surveillance.
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