Evidence map›Paper›PMID 27809830›Full record

ArticleBMC complementary and alternative medicine2016

The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity.

Chia-Chuan Chang, Wei Yuan, Hsiao-Yuh Roan, Jia-Ling Chang, Hsiu-Chen Huang, Yu-Ching Lee, Huey Jen Tsay, Hui-Kang Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC complementary and alternative medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
5.5field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 22 citations in OpenAlex.

  1. Anti-Diabetic Potential of Corn (Preventive nutrition and food science · 2026
    Review
  2. Article
  3. Article
  4. Article
  5. Oxidative Stress in Pancreatic Beta Cell Regeneration.Oxidative medicine and cellular longevity · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 6 institutions in 1 country.

Chia-Chuan ChangSchool of Pharmacy, College of Medicine, National Taiwan University, Taipei, Taiwan, ROC.
Wei YuanDepartment of Psychiatry, Cheng Hsin General Hospital, Taipei, Taiwan, ROC.
Hsiao-Yuh RoanDepartment of Life Sciences and Institute of Genome Sciences, National Yang-Ming University, Taipei, Taiwan, ROC.
Jia-Ling ChangDivision of Basic Chinese Medicine, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan, ROC.
Hsiu-Chen HuangDepartment of Applied Science, National Hsinchu University of Education, Hsinchu, Taiwan, ROC.
Yu-Ching LeeThe Center of Translational Medicine, Taipei Medical University, Taipei, Taiwan, ROC.
Huey Jen TsayInstitute of Neuroscience, Brain Research Centre, National Yang-Ming University, Taipei, Taiwan, ROC.
Hui-Kang LiuDivision of Basic Chinese Medicine, National Research Institute of Chinese Medicine, Ministry of Health and Welfare, Taipei, Taiwan, ROC. hk.liu@nricm.edu.tw.
National Yang Ming Chiao Tung University · TWTaipei Medical University · TWCheng Hsin General Hospital · TWMinistry of Health and Welfare · TWNational Hsinchu University of Education · TWNational Taiwan University · TW

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure.

methodsCorn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test.

resultsFour corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds.

conclusionsWe concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.

Indexed as

AcetatesAnimalsAntioxidantsCell LineCell SurvivalFemaleGlycation End Products, AdvancedHydrogen PeroxideMaleMiceOxidative StressPlant ExtractsRatsReactive Oxygen SpeciesZea maysAcetatesAntioxidantsethyl acetateGlycation End Products, AdvancedHydrogen PeroxidePlant ExtractsReactive Oxygen SpeciesAdvanced glycation end productsGlucotoxicityMethylglyoxalReactive oxygen speciesStigmata Maydis (corn silk)β-cell failure

Identifiers

PMID27809830
PMCPMC5294807
OpenAlexW2547582070

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.