ArticleAsian-Australasian journal of animal sciences2017
Myotube differentiation in clustered regularly interspaced short palindromic repeat/Cas9-mediated MyoD knockout quail myoblast cells.
Article in Asian-Australasian journal of animal sciences, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 13 citations in OpenAlex.
- Genome-wide characteristics and potential functions of circular RNAs from the embryo muscle development in Chengkou mountain chicken.Frontiers in veterinary science · 2024Article
- - Invited Review - Gene-editing techniques and their applications in livestock and beyond.Animal bioscience · 2023Article
- Transcriptomic Analysis ofGenes · 2021Article
- Necessity for Validation of Effectiveness of Selected Guide RNA In Silico for Application of CRISPR/Cas9.Molecular biotechnology · 2021Article
- Muscle differentiation induced by p53 signaling pathway-related genes in myostatin-knockout quail myoblasts.Molecular biology reports · 2020Article
- Functional analyses of miRNA-146b-5p during myogenic proliferation and differentiation in chicken myoblasts.BMC molecular and cell biology · 2020Article
- Muscle differentiation induced up-regulation of calcium-related gene expression in quail myoblasts.Asian-Australasian journal of animal sciences · 2018Article
- CRISPR-Trap: a clean approach for the generation of gene knockouts and gene replacements in human cells.Molecular biology of the cell · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveIn the livestock industry, the regulatory mechanisms of muscle proliferation and differentiation can be applied to improve traits such as growth and meat production. We investigated the regulatory pathway of MyoD and its role in muscle differentiation in quail myoblast cells.
methodsThe
resultsThe mutation efficiency was 73.3% in the mixed population, and from this population we were able to establish two QM7 MyoD knockout subline (MyoD KO QM7#4) through single cell pick-up and expansion. In the undifferentiated condition, paired box 7 expression in MyoD KO QM7#4 cells was not significantly different from regular QM7 (rQM7) cells. During differentiation, however, myotube formation was dramatically repressed in MyoD KO QM7#4 cells. Moreover, myogenic differentiation-specific transcripts and proteins were not expressed in MyoD KO QM7#4 cells even after an extended differentiation period. These results indicate that MyoD is critical for muscle differentiation. Furthermore, we analyzed the global regulatory interactions by RNA sequencing during muscle differentiation.
conclusionWith CRISPR/Cas9-mediated genomic editing, single cell-derived sublines with a specific knockout gene can be adapted to various aspects of basic research as well as in functional genomics studies.
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