ReviewJournal of pathology and translational medicine2016
Immunohistochemistry for Pathologists: Protocols, Pitfalls, and Tips.
Review in Journal of pathology and translational medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 234 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
234 citing papers in PubMed, 1 synthesis or guideline pooled it.
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- Comparative effectiveness of levofloxacin versus third-generation cephalosporins and predictors of treatment outcomes in upper respiratory tract scleroma: a tertiary center experience.Scientific reports · 2026Trial
- Chikungunya Virus Infection in Paraffin-Embedded Tissue: Analysis of Histological Alterations and Viral Detection by Immunohistochemistry.Methods in molecular biology (Clifton, N.J.) · 2027Article
- A Comparative Analysis of the Efficacy of CD117 and Special Stains as Indicators of Mechanical Asphyxial Deaths.Annals of African medicine · 2026Article
- Digital Quantification of Membrane DAB Immunohistochemical Staining in FFPE Cervical Cancer Tissues Using an Open-Source CellProfiler Pipeline.Bio-protocol · 2026Article
- DNA methylation profiling enables subclassification of mucinous ovarian carcinoma and distinguishes it from extraovarian mucinous metastases.Cell reports. Medicine · 2026Article
- Assessment of c-Kit immunohistochemical expression in cutaneous basal and squamous cell carcinomas.Scientific reports · 2026Article
- A novel rat model for investigating erectile function after nerve-sparing radical prostatectomy.International journal of impotence research · 2026Article
- Primary Intracranial CIC-Rearranged Sarcoma With Prior History of B-Cell ALL: A Case Report.Neuropathology : official journal of the Japanese Society of Neuropathology · 2026Article
- Application of Cumulative Threshold Approaches to Tissue Iron Measurement on Histochemical or Synchrotron X‑ray Fluorescence Platforms.Chemical & biomedical imaging · 2026Article
- A clear cell sarcoma tumor of the finger with an unusual presentation misdiagnosed as a glomus tumor in an adolescent patient: A case report.Oncology letters · 2026Article
- UNICORN: a deep learning model for integrating multi-stain data in histopathology.NPJ digital medicine · 2026Article
- Quantifying PD1 Saturation by PDL1 in Tumor Tissue Using a Novel RNA Aptamer-Based Assay.International journal of molecular sciences · 2026Article
- Article
- Morpho-functional plasticity of tumour-associated macrophages: linking cell shape, ultrastructure, and immune regulation in cancer.Clinical and experimental medicine · 2026Review
- Enhanced recovery of high-quality DNA from limited FFPE tissue for advancing cancer genomics.Scientific reports · 2026Article
- CD8 immunoPET imaging to stratify response and guide combination immunotherapy and radiation in triple negative breast cancer.Breast cancer research : BCR · 2026Article
- Comprehensive Overview of Gastric Cancer Immunohistochemistry: Key Biomarkers, Advanced Detection Methods, and Perspectives.Medicina (Kaunas, Lithuania) · 2026Review
- Enhanced terahertz near-field nanoscopic imaging for label-free assessment of ovarian cancer cells: a preliminary study.Biomedical optics express · 2026Article
- Review
174 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment. Although there has been a considerable progress in automation and standardization of IHC, there are still many things to be considered in proper optimization and appropriate interpretation. In this review, we aim to provide possible pitfalls and useful tips for practicing pathologists and residents in pathology training. First, general procedure of IHC is summarized, followed by pitfalls and tips in each step and a summary of troubleshooting. Second, ways to an accurate interpretation of IHC are discussed, with introduction to general quantification and analysis methods. This review is not intended to provide complete information on IHC, but to be used as a basic reference for practice and publication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.