ArticleNature protocols2016
A microfluidics-based method for measuring neuronal activity in Drosophila chemosensory neurons.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 27 citations in OpenAlex.
- Food hardness preference reveals multisensory contributions of fly larval gustatory organs in behaviour and physiology.PLoS biology · 2025Article
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- Porous Structural Microfluidic Device for Biomedical Diagnosis: A Review.Micromachines · 2023Review
- An optofluidic platform for interrogating chemosensory behavior and brainwide neural representation in larval zebrafish.Nature communications · 2023Article
- Taste sensing and sugar detection mechanisms ineLife · 2021Article
- Article
- Collective behaviors of Drosophila-derived retinal progenitors in controlled microenvironments.PloS one · 2019Article
- Tools to reverse-engineer multicellular systems: case studies using the fruit fly.Journal of biological engineering · 2019Review
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- Article
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Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Monitoring neuronal responses to defined sensory stimuli is a powerful and widely used approach for understanding sensory coding in the nervous system. However, providing precise, stereotypic and reproducible cues while concomitantly recording neuronal activity remains technically challenging. Here we describe the fabrication and use of a microfluidics system that allows precise temporally restricted stimulation of Drosophila chemosensory neurons with an array of different chemical cues. The system can easily be combined with genetically encoded calcium sensors, and it can measure neuronal activity at single-cell resolution in larval sense organs and in the proboscis or leg of the adult fly. We describe the design of the master mold, the production of the microfluidic chip and live imaging using the calcium sensor GCaMP, expressed in distinct types of Drosophila chemosensory neurons. Fabrication of the master mold and microfluidic chips requires basic skills in photolithography and takes ∼2 weeks; the same devices can be used repeatedly over several months. Flies can be prepared for measurements in minutes and imaged for up to 1 h.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.