Evidence map›Paper›PMID 27809315›Full record

ArticleNature protocols2016

Rapid identification of causal mutations in tomato EMS populations via mapping-by-sequencing.

Virginie Garcia, Cécile Bres, Daniel Just, Lucie Fernandez, Fabienne Wong Jun Tai, Jean-Philippe Mauxion, Marie-Christine Le Paslier, Aurélie Bérard, Dominique Brunel, Koh Aoki and 4 more

Abstract read
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In one paragraph

Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
15.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 84 citations in OpenAlex.

  1. Article
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  4. Review
  5. Article
  6. An Ethyl Methanesulfonate-InducedPlants (Basel, Switzerland) · 2024
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 4 countries.

Virginie GarciaInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.
Cécile BresInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.ORCID http://orcid.org/0000-0001-6460-4637
Daniel JustInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.
Lucie FernandezInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.
Fabienne Wong Jun TaiInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.
Jean-Philippe MauxionInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.
Marie-Christine Le PaslierInstitut National de la Recherche Agronomique US1279 Etude du Polymorphisme des Génomes Végétaux, CEA-Institut de Génomique-CNG, Evry, France.
Aurélie BérardInstitut National de la Recherche Agronomique US1279 Etude du Polymorphisme des Génomes Végétaux, CEA-Institut de Génomique-CNG, Evry, France.
Dominique BrunelInstitut National de la Recherche Agronomique US1279 Etude du Polymorphisme des Génomes Végétaux, CEA-Institut de Génomique-CNG, Evry, France.
Koh AokiGraduate School of Life and Environmental Sciences, Osaka Prefecture University, Osaka, Japan.
Saleh AlseekhMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.
Alisdair R FernieMax-Planck-Institut für Molekulare Pflanzenphysiologie, Potsdam-Golm, Germany.
Paul D FraserSchool of Biological Sciences, Royal Holloway University of London, Egham, UK.
Christophe RothanInstitut National de la Recherche Agronomique and Université de Bordeaux, Unité Mixte de Recherche 1332 Biologie du Fruit et Pathologie, Villenave d'Ornon, France.ORCID http://orcid.org/0000-0002-6831-2823
Université de Bordeaux · FRCommissariat à l'Énergie Atomique et aux Énergies Alternatives · FRMax Planck Institute of Molecular Plant Physiology · DEOsaka Prefecture University · JPRoyal Holloway University of London · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The tomato is the model species of choice for fleshy fruit development and for the Solanaceae family. Ethyl methanesulfonate (EMS) mutants of tomato have already proven their utility for analysis of gene function in plants, leading to improved breeding stocks and superior tomato varieties. However, until recently, the identification of causal mutations that underlie particular phenotypes has been a very lengthy task that many laboratories could not afford because of spatial and technical limitations. Here, we describe a simple protocol for identifying causal mutations in tomato using a mapping-by-sequencing strategy. Plants displaying phenotypes of interest are first isolated by screening an EMS mutant collection generated in the miniature cultivar Micro-Tom. A recombinant F

Indexed as

MutationDNA Mutational AnalysisEthyl MethanesulfonateGenetic VariationSolanum lycopersicumTime FactorsEthyl Methanesulfonate

Identifiers

PMID27809315
OpenAlexW2547119088

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.