ArticleNature protocols2016
Large-scale production and isolation of Candida biofilm extracellular matrix.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Biofilm-mediated antibiotic tolerance in bacterial pathogens: Integrated molecular networks and novel therapeutic avenues.Virulence · 2026Review
- Integrated characterization of extracellular matrix remodeling in Candida parapsilosis biofilms by surface-enhanced raman spectroscopy, protein analysis, ultrastructural imaging and multivariate analysis.World journal of microbiology & biotechnology · 2026Article
- In-situ revitalizing end-of-life MBR membranes via a curtain-type dynamic membrane process.Nature communications · 2026Article
- Article
- Secretion of the fungal toxin candidalysin is dependent on conserved precursor peptide sequences.Nature microbiology · 2024Article
- Candida albicans extracellular vesicles trigger type I IFN signalling via cGAS and STING.Nature microbiology · 2024Article
- The human pathobiontProceedings of the National Academy of Sciences of the United States of America · 2022Article
- A common vesicle proteome drives fungal biofilm development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Production and Isolation of the Candida Species Biofilm Extracellular Matrix.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Coordination of fungal biofilm development by extracellular vesicle cargo.Nature communications · 2021Article
- A Label-Free Cellular Proteomics Approach to Decipher the Antifungal Action of DiMIQ, a Potent Indolo[2,3-International journal of molecular sciences · 2020Article
- The Role ofFrontiers in microbiology · 2020Article
- Extraction of Extracellular Matrix in Static and DynamicFrontiers in microbiology · 2019Article
- Article
- BIOF-HILO Assay: A New MALDI-TOF Mass Spectrometry Based Method for Discriminating Between High- and Low-Biofilm-ProducingFrontiers in microbiology · 2019Article
- The Significance of Lipids to Biofilm Formation inJournal of fungi (Basel, Switzerland) · 2018Review
- Candida albicans biofilm-induced vesicles confer drug resistance through matrix biogenesis.PLoS biology · 2018Article
- Conservation and Divergence in themBio · 2018Article
- TheJournal of fungi (Basel, Switzerland) · 2017Article
- Proteomics of Staphylococcus aureus biofilm matrix in a rat model of orthopedic implant-associated infection.PloS one · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The extracellular matrix of biofilm is unique to the biofilm lifestyle, and it has key roles in community survival. A complete understanding of the biochemical nature of the matrix is integral to the understanding of the roles of matrix components. This knowledge is a first step toward the development of novel therapeutics and diagnostics to address persistent biofilm infections. Many of the assay methods needed for refined matrix composition analysis require milligram amounts of material that is separated from the cellular components of these complex communities. The protocol described here explains the large-scale production and isolation of the Candida biofilm extracellular matrix. To our knowledge, the proposed procedure is the only currently available approach in the field that yields milligram amounts of biofilm matrix. This procedure first requires biofilms to be seeded in large-surface-area roller bottles, followed by cell adhesion and biofilm maturation during continuous movement of the medium across the surface of the rotating bottle. The formed matrix is then separated from the entire biomass using sonication, which efficiently removes the matrix without perturbing the fungal cell wall. Subsequent filtration, dialysis and lyophilization steps result in a purified matrix product sufficient for biochemical, structural and functional assays. The overall protocol takes ∼11 d to complete. This protocol has been used for Candida species, but, using the troubleshooting guide provided, it could be adapted for other fungi or bacteria.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.