ArticleNature protocols2016
Labeling cellular structures in vivo using confined primed conversion of photoconvertible fluorescent proteins.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Spinal cord regeneration deploys cell-type specific developmental and non-developmental strategies to restore neuron diversity.Nature communications · 2026Article
- Structure-Based Understanding of CuBiosensors · 2025Review
- Long-term tracing of individual human neural cells using multiphoton microscopy and photoconvertible polymer capsules.Journal of the Royal Society, Interface · 2024Article
- Diving into the zebrafish brain: exploring neuroscience frontiers with genetic tools, imaging techniques, and behavioral insights.Frontiers in molecular neuroscience · 2024Review
- A decrease in Fkbp52 alters autophagosome maturation and A152T-tau clearanceFrontiers in cellular neuroscience · 2024Article
- Improving Photocleavage Efficiency of Photocleavable Protein for Antimicrobial Peptide Histatin 1 Expression.Protein and peptide letters · 2024Article
- Implantable photonic neural probes with 3D-printed microfluidics and applications to uncaging.Frontiers in neuroscience · 2023Article
- Primed Track: Reliable Volumetric Single-cell Tracking and Lineage Tracing of Living Specimen with Dual-labeling Approaches.Bio-protocol · 2020Article
- A biphasic growth model for cell pole elongation in mycobacteria.Nature communications · 2020Article
- Article
- Continuous addition of progenitors forms the cardiac ventricle in zebrafish.Nature communications · 2018Article
- Following Endocardial Tissue Movements via Cell Photoconversion in the Zebrafish Embryo.Journal of visualized experiments : JoVE · 2018Article
- Combining Primed Photoconversion and UV-Photoactivation for Aberration-Free, Live-Cell Compliant Multi-Color Single-Molecule Localization Microscopy Imaging.International journal of molecular sciences · 2017Article
- Quantifying transcription factor-DNA binding in single cells in vivo with photoactivatable fluorescence correlation spectroscopy.Nature protocols · 2017Article
- The rise of photoresponsive protein technologies applicationsF1000Research · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The application of green-to-red photoconvertible fluorescent proteins (PCFPs) for in vivo studies in complex 3D tissue structures has remained limited because traditional near-UV photoconversion is not confined in the axial dimension, and photomodulation using axially confined, pulsed near-IR (NIR) lasers has proven inefficient. Confined primed conversion is a dual-wavelength continuous-wave (CW) illumination method that is capable of axially confined green-to-red photoconversion. Here we present a protocol to implement this technique with a commercial confocal laser-scanning microscope (CLSM); evaluate its performance on an in vitro setup; and apply primed conversion for in vivo labeling of single cells in developing zebrafish and mouse preimplantation embryos expressing the green-to-red photoconvertible protein Dendra2. The implementation requires a basic understanding of laser-scanning microscopy, and it can be performed within a single day once the required filter cube is manufactured.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.