ArticleNature protocols2016
Laser microdissection of tomato fruit cell and tissue types for transcriptome profiling.
Article in Nature protocols, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 44 citations in OpenAlex.
- Transcriptomic and metabolomic changes associated with the induction and initiation of juice sacs in citrus fruit.Planta · 2026Article
- Live Cell Isolation by Laser Capture Microdissection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Co-extraction of Small RNAs and Total RNA from Arbuscular Mycorrhizal Roots Using Laser Microdissection.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Use of polyethylene glycol as an alternative to optimal cutting temperature medium in freeze sectioning for plant histochemical studies.Protoplasma · 2025Article
- Developing a simple and rapid method for cell-specific transcriptome analysis through laser microdissection: insights from citrus rind with broader implications.Plant methods · 2024Article
- Editorial: Transcriptomics of fruit growth, development and ripening.Frontiers in plant science · 2024Article
- The calcium-mediated homogalacturonan pectin complexation in cell walls contributes the firmness increase in loquat fruit during postharvest storage.Journal of advanced research · 2023Article
- Laser Microdissection of Woody and Suberized Plant Tissues for RNA-Seq Analysis.Molecular biotechnology · 2023Article
- Advances and Opportunities in Single-Cell Transcriptomics for Plant Research.Annual review of plant biology · 2021Review
- The Landscape of RNA-Protein Interactions in Plants: Approaches and Current Status.International journal of molecular sciences · 2021Review
- Laser Microdissection of Specific Stem-Base Tissue Types from Olive Microcuttings for Isolation of High-Quality RNA.Biology · 2021Article
- FIS1 encodes a GA2-oxidase that regulates fruit firmness in tomato.Nature communications · 2020Article
- Long-Term Impact of Chemical and Alternative Fungicides Applied to GrapevineInternational journal of molecular sciences · 2020Article
- Dissection of complex traits of tomato in the post-genome era.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2020Review
- A rapid preparation procedure for laser microdissection-mediated harvest of plant tissues for gene expression analysis.Plant methods · 2019Article
- Spatially resolved transcriptomics reveals plant host responses to pathogens.Plant methods · 2019Article
- A protocol for laser microdissection (LMD) followed by transcriptome analysis of plant reproductive tissue in phylogenetically distant angiosperms.Plant methods · 2019Article
- High-resolution spatiotemporal transcriptome mapping of tomato fruit development and ripening.Nature communications · 2018Article
- The Tomato Expression Atlas.Bioinformatics (Oxford, England) · 2017Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
This protocol enables transcriptome profiling of specific cell or tissue types that are isolated from tomato using laser microdissection (LM). To prepare tissue for LM, fruit samples are first fixed in optimal cutting temperature (OCT) medium and frozen in molds. The tissue is then sectioned using a cryostat before being dissected using an LM instrument. The RNAs contained in the harvested cells are purified and subjected to two rounds of amplification to yield sufficient quantities of RNA to generate cDNA libraries. Unlike several other techniques that are used to isolate specific cell types, LM has the advantage of being readily applied to any plant species without having to generate transgenic plants. Using the protocols described here, LM-mediated cell-type transcriptomic analysis of two samples requires ∼8 d from tissue harvest to RNA sequencing (RNA-seq), whereas each additional sample, up to a total of 12 samples, requires ∼1 additional day for the LM step. RNA obtained using this method has been successfully used for deep-coverage transcriptome profiling, which is a particularly effective strategy for identifying genes that are differentially expressed between cell or tissue types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.