ArticleInternational journal of legal medicine2017
Characterization of degradation and heterozygote balance by simulation of the forensic DNA analysis process.
Article in International journal of legal medicine, 2017. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 28 citations in OpenAlex.
- Understanding the mechanistic interaction between DNA and polystyrene microplastic and the effect of microplastic on forensic DNA analysis.International journal of legal medicine · 2026Article
- Beyond the Core: The Role of Supplementary Short Tandem Repeats in Forensic Genetics.International journal of molecular sciences · 2026Review
- Exploration of identifying individual tumor tissue based on probabilistic model.Frontiers in oncology · 2024Article
- Systematic selections and forensic application evaluations of 111 individual identification SNPs in the Chinese Inner Mongolia Manchu group.Frontiers in genetics · 2022Article
- A Review of Probabilistic Genotyping Systems:Genes · 2021Review
- Towards developing forensically relevant single-cell pipelines by incorporating direct-to-PCR extraction: compatibility, signal quality, and allele detection.International journal of legal medicine · 2021Article
- Degradation of nuclear and mitochondrial DNA after γ-irradiation and its effect on forensic genotyping.Forensic science, medicine, and pathology · 2020Article
- Alternatives to amelogenin markers for sex determination in humans and their forensic relevance.Molecular biology reports · 2020Review
- The importance of forensic storage support: DNA quality from 11-year-old saliva on FTA cards.International journal of legal medicine · 2019Article
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Authors and funding
3 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Simulation experiments were used to show the impact of varying extraction efficiency, aliquot proportion, and PCR efficiency on the heterozygote balance of a range of diploid and haploid cells. Reducing either parameters introduces variance. It is well-known that the variance in heterozygote balance increases as the amount of DNA is reduced. Surprisingly the distribution is in fact diamond shaped - the variance start to decrease at very low amounts of DNA. Simulations suggest that pristine diluted DNA is an acceptable approximation in validations to infer heterozygote balance. However, the difference in distribution of the variance between diploid and haploid cell types may, under some circumstances, need to be considered in statistical models. Finally, we exemplify how simulations can be used to predict the outcome of PCR for degraded samples. Visualizing the predicted DNA profile as an electropherogram can help to identify the best approach for sample processing.
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