ArticleBiophysical journal2016
Measuring Cell Viscoelastic Properties Using a Microfluidic Extensional Flow Device.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed.
- A geometric-surface PDE model for cell-nucleus translocation through confinement.PLoS computational biology · 2026Article
- Resilient Biophysical Phenotype of Memory CD4Immunology · 2026Article
- Active Force Dynamics in Red Blood Cells Under Non-Invasive Optical Tweezers.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- At the Pointy End of Mechanobiology: AFM for Transient Biomechanical Analysis.Advanced healthcare materials · 2025Review
- Conditions for a microfluidic creep experiment for microparticles using a cross-slot extensional flow device.Biomicrofluidics · 2025Article
- Real-time viscoelastic deformability cytometry: High-throughput mechanical phenotyping of liquid and solid biopsies.Science advances · 2024Article
- Single-cell electro-mechanical shear flow deformability cytometry.Microsystems & nanoengineering · 2024Article
- Recent Advances of PDMS In Vitro Biomodels for Flow Visualizations and Measurements: From Macro to Nanoscale Applications.Micromachines · 2024Review
- A miniaturized wash-free microfluidic assay for electrical impedance-based assessment of red blood cell-mediated microvascular occlusion.Biosensors & bioelectronics · 2024Article
- Elasticity of Carrier Fluid: A Key Factor Affecting Mechanical Phenotyping in Deformability Cytometry.Micromachines · 2024Article
- Rapid single-cell physical phenotyping of mechanically dissociated tissue biopsies.Nature biomedical engineering · 2023Article
- A reliable elasticity sensing method for analysis of cell entosis using microfluidic cytometer.Biomedical engineering letters · 2023Article
- Non-contact microfluidic analysis of the stiffness of single large extracellular vesicles from IDH1-mutated glioblastoma cells.Advanced materials technologies · 2023Article
- Microfluidic techniques for mechanical measurements of biological samples.Biophysics reviews · 2023Review
- Article
- Pilot study on the influence of acute alcohol exposure on biophysical parameters of leukocytes.Frontiers in molecular biosciences · 2023Article
- Article
- Blood Particulate Analogue Fluids: A Review.Materials (Basel, Switzerland) · 2021Review
- Rapid viscoelastic changes are a hallmark of early leukocyte activation.Biophysical journal · 2021Article
- Non-invasive acquisition of mechanical properties of cells via passive microfluidic mechanisms: A review.Biomicrofluidics · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
The quantification of cellular mechanical properties is of tremendous interest in biology and medicine. Recent microfluidic technologies that infer cellular mechanical properties based on analysis of cellular deformations during microchannel traversal have dramatically improved throughput over traditional single-cell rheological tools, yet the extraction of material parameters from these measurements remains quite complex due to challenges such as confinement by channel walls and the domination of complex inertial forces. Here, we describe a simple microfluidic platform that uses hydrodynamic forces at low Reynolds number and low confinement to elongate single cells near the stagnation point of a planar extensional flow. In tandem, we present, to our knowledge, a novel analytical framework that enables determination of cellular viscoelastic properties (stiffness and fluidity) from these measurements. We validated our system and analysis by measuring the stiffness of cross-linked dextran microparticles, which yielded reasonable agreement with previously reported values and our micropipette aspiration measurements. We then measured viscoelastic properties of 3T3 fibroblasts and glioblastoma tumor initiating cells. Our system captures the expected changes in elastic modulus induced in 3T3 fibroblasts and tumor initiating cells in response to agents that soften (cytochalasin D) or stiffen (paraformaldehyde) the cytoskeleton. The simplicity of the device coupled with our analytical model allows straightforward measurement of the viscoelastic properties of cells and soft, spherical objects.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.